Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pT-E-pY Motif) Antikörper

Antigen: Mitogen-Activated Protein Kinase 1/3 (MAPK1/3)

Synonyme: erk1/2, sapk4, prkm13, p38delta

Epitope: pT-E-pY Motif

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN153114

Produktbeschreibung

Weitere Bezeichnung: ERK1 / ERK2 (pT-E-pY Motif)

Gen-ID: 5595, 26417, 50689

Immunogen: Synthetic phospholyrated peptide (KLH coupled) corresponding to residues aroundThr202/Tyr204 of human p44 MAP kinase (Erk1 and Erk2).

Format: Serum

Beschreibung: ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activatedprotein kinase) has two closely related isoforms of 44 kDa and 42 kDa, respectively.These kinases belong to a family of serine/threonine kinases that are activated upontreatment of cells with a large variety of stimuli including mitogens, hormones, growthfactors, cytokines, and bioactive peptides. Cell stimulation induces the activation of asignaling cascade, the downstream effects of which have been linked to the regulationof cell growth and differentiation as well as the cytoskeleton. ERK1 and ERK2 arephosphorylated within the activation loop on both a Threonine and a Tyrosine residue(within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevatingthe activity of ERK1&2. Alternate Names: anti-ERT1 antibody, anti-Extracellular signal regulated kinase 1 antibody,anti-Extracellular signal regulated kinase 2 antibody, anti-MAP kinase 2 antibody,anti-MAPK 2 antibody, anti-MAPK 3 antibody, anti-Mitogen activated protein kinase 1antibody, anti-Mitogen activated protein kinase 3 antibody, anti-p42MAPK antibody,anti-p44ERK1 antibody, anti-p44MAPK antibody, anti-PRKM 1 antibody, anti-PRKM 2antibody, anti-PRKM 3 antibody, anti-Protein kinase mitogen activated 1 antibody,anti-Protein kinase mitogen activated 2 antibody, anti-Protein kinase mitogen activated 3antibody, anti-Protein tyrosine kinase ERK 2 antibody.

Spezifität: This antibody recognises ERK1 phosphorylated at Thr202 and ERK1 phosphorylated atTyr204.Phosphorylated:Thr202/Tyr204. Species Reactivity: Cross-reacts with Human, Mouse and Rat. Not yet tested in other species. Localization: Cell Membrane.

Anwendungen

Applikationshinweise: Suggested working dilutions: Western Blot 1/5000. Positive Controls: NIH3T3 cell lysates stimulated with PDGF.

Konzentration: 1 mg/ml

Buffer: 50% Glycerol, PBS

Lagerung: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Forschungsgebiet: Signaltransduktion, Inflammation, Phospho-Antikörper

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunohistochemical staining of paraffin-embedded human lung cancer tissue using Zymed Rb anti-phospho-ERK1+2 (Thr202/Tyr204)

Publikationen

Publikationen: Kolch: "Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions." in: The Biochemical journal, Vol. 351 Pt 2, pp. 289-305, 2001 (PubMed).

Johnson, Lapadat: "Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases." in: Science (New York, N.Y.), Vol. 298, Issue 5600, pp. 1911-2, 2002 (PubMed).

Smalley: "A pivotal role for ERK in the oncogenic behaviour of malignant melanoma?" in: International journal of cancer. Journal international du cancer, Vol. 104, Issue 5, pp. 527-32, 2003 (PubMed).

Koleganova, Piecha, Ritz et al.: "Calcitriol ameliorates capillary deficit and fibrosis of the heart in subtotally nephrectomized rats." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 24, Issue 3, pp. 778-87, 2009 (PubMed).