Survivin Antikörper (BIRC5) (Cytoplasmic Form)

Details for Product anti-BIRC5 Antibody No. ABIN153038, Anbieter: Anmelden zum Anzeigen
Antigen
  • bir5a
  • survivin
  • LOC398389
  • BIRC5
  • BIR1
  • Su2
  • Survivin
  • Survivin1
  • XBIR1
  • api4
  • epr-1
  • svv1
  • xSurvivin1
  • xsurv
  • SIX
  • Su1
  • svv2
  • birc5
  • xSurvivin
  • xSurvivin2
  • AAC-11
  • Api4
  • TIAP
  • survivin40
  • baculoviral IAP repeat-containing 5a
  • survivin
  • baculoviral IAP repeat-containing 5
  • baculoviral IAP repeat-containing 5, gene 1
  • baculoviral IAP repeat containing 5
  • birc5a
  • LOC398389
  • BIRC5
  • birc5.1
  • birc5.2
  • AaeL_AAEL012446
  • CpipJ_CPIJ001663
  • CpipJ_CPIJ002689
  • CpipJ_CPIJ006694
  • Birc5
Alternativen
anti-Ratte (Rattus) Survivin Antikörper für Blocking Peptide
Epitop
Cytoplasmic Form
65
25
23
18
18
16
15
15
13
7
6
6
5
5
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
425
166
162
35
20
19
12
4
2
2
1
Wirt
Maus
294
138
16
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser Survivin Antikörper ist unkonjugiert
24
17
16
13
13
13
12
11
9
9
8
8
6
5
5
5
5
3
3
3
2
2
2
2
2
2
2
1
Applikation
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
346
202
179
158
147
123
106
33
32
26
7
5
3
3
3
3
2
2
2
2
1
1
1
Optionen
Hersteller
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Immunogen Full length recombinant human Survivin [UniProt# O15392]
Klon 60-11
Isotyp IgG2a kappa
Spezifität This antibody is specific for the cytoplasmic form of Survivin.
Reinigung Protein G purified
Andere Bezeichnung Survivin (BIRC5 Antibody Abstract)
Hintergrund Gene Symbol: BIRC5
Molekulargewicht Theoretical MW: 16.5 kDa
Gen-ID 332
UniProt O15392
Forschungsgebiet Neurology, Apoptosis/Necrosis, Glia marker, Cancer
Pathways Nuclear Hormone Receptor Binding
Applikationshinweise Western Blot 1:1000, Flow Cytometry 1 μg/mL, ELISA, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-FrozenThis Survivin (60.11) antibody is useful for Immunocytochemistry (PMID: 21976248)/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. By WB, this antibody recognizes a band at ~16.5 kDa representing Survivin. Flow Cytometry and ELISA were reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for Survivin Antibody Immunohistochemistry - FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.9 mg/mL
Buffer PBS
Buffer contains: 0.1 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung -80 °C,-20 °C
Informationen zur Lagerung Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Survivin Antikörper (BIRC5) (Cytoplasmic Form) (ABIN153038) Staing of ovary, cancer using ABIN153038.
Western Blotting (WB) image for anti-Survivin Antikörper (BIRC5) (Cytoplasmic Form) (ABIN153038) Detection of Survivin in HeLa whole cell extract using ABIN153038.
Western Blotting (WB) image for anti-Survivin Antikörper (BIRC5) (Cytoplasmic Form) (ABIN153038) Western Blot: Survivin Antibody (60.11) [ABIN153038] - Detected in rat aorta smooth m...
 image for anti-Survivin Antikörper (BIRC5) (Cytoplasmic Form) (ABIN153038) Flow (Intracellular): Survivin Antibody (60.11) [ABIN153038] - An intracellular stain...
Flow Cytometry (FACS) image for anti-Survivin Antikörper (BIRC5) (Cytoplasmic Form) (ABIN153038) Flow Cytometry: Survivin Antibody (60.11) [ABIN153038] - Staining of NTERA-2 cells us...
Western Blotting (WB) image for anti-Survivin Antikörper (BIRC5) (Cytoplasmic Form) (ABIN153038) Western Blot: Survivin Antibody (60.11) [ABIN153038] - Cells were transfected with th...
Immunofluorescence (IF) image for anti-Survivin Antikörper (BIRC5) (Cytoplasmic Form) (ABIN153038) Immunocytochemistry/Immunofluorescence: Survivin Antibody (60.11) [ABIN153038] - Dete...
Immunofluorescence (IF) image for anti-Survivin Antikörper (BIRC5) (Cytoplasmic Form) (ABIN153038) Immunocytochemistry/Immunofluorescence: Survivin Antibody (60.11) [ABIN153038] - IHeL...
Produkt verwendet in: Bae, Kim, Kim, Cheong, Ryu, Han: "Correlation of survivin and B-cell lymphoma 2 expression with pathological malignancy and anti-apoptotic properties of glial cell tumors." in: Biomedical reports, Vol. 6, Issue 4, pp. 396-400, 2017 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Dheekollu, Malecka, Wiedmer, Delecluse, Chiang, Altieri, Messick, Lieberman: "Carcinoma-risk variant of EBNA1 deregulates Epstein-Barr Virus episomal latency." in: Oncotarget, Vol. 8, Issue 5, pp. 7248-7264, 2017 (PubMed). Von den Autoren verwendete Methode: Proximity Ligation Assay (PLA)

Asuncion Valenzuela, Castro, Gonda, Diaz Osterman, Jutzy, Aspe, Khan, Neidigh, Wall: "Cell death in response to antimetabolites directed at ribonucleotide reductase and thymidylate synthase." in: OncoTargets and therapy, Vol. 8, pp. 495-507, 2015 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Kusner, Ciesielski, Marx, Kaminski, Fenstermaker: "Survivin as a potential mediator to support autoreactive cell survival in myasthenia gravis: a human and animal model study." in: PLoS ONE, Vol. 9, Issue 7, pp. e102231, 2014 (PubMed). (Probematerial (Species): Human). Weitere Details: Flow Cytometry

Kapinas, Kim, Mandeville, Martin-Buley, Croce, Lian, van Wijnen, Stein, Altieri, Stein: "microRNA-mediated survivin control of pluripotency." in: Journal of cellular physiology, Vol. 230, Issue 1, pp. 63-70, 2014 (PubMed). (Probematerial (Species): Human).

Rho, Lampe: "High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays." in: Journal of proteome research, Vol. 12, Issue 5, pp. 2311-20, 2013 (PubMed). (Probematerial (Species): Human).

Femia, Salvianti, Luceri, Dolara, Salvadori, Pinzani, Caderni: "Sustained proliferation and resistance to apoptosis after a cytotoxic insult are early alterations in rat colon carcinogenesis." in: International journal of cancer, Vol. 131, Issue 3, pp. 529-36, 2012 (PubMed). (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Sakao, Desineni, Hahm, Singh: "Phenethyl isothiocyanate suppresses inhibitor of apoptosis family protein expression in prostate cancer cells in culture and in vivo." in: The Prostate, Vol. 72, Issue 10, pp. 1104-16, 2012 (PubMed). Weitere Details: Western Blotting,Immunohistochemistry

Keerthivasan, Liu, Gump, Dowdy, Wickrema, Crispino: "A novel role for survivin in erythroblast enucleation." in: Haematologica, Vol. 97, Issue 10, pp. 1471-9, 2012 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunocytochemistry,Western Blotting,Immunofluorescence

Zhang, Wang, Liu, Zhang, Xia, Zhang, Wu, Jiang, Chen, Wu, Zha, Yu, Kong: "DNA and adenovirus tumor vaccine expressing truncated survivin generates specific immune responses and anti-tumor effects in a murine melanoma model." in: Cancer immunology, immunotherapy : CII, Vol. 61, Issue 10, pp. 1857-67, 2012 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Khan, Jutzy, Valenzuela, Turay, Aspe, Ashok, Mirshahidi, Mercola, Lilly, Wall: "Plasma-derived exosomal survivin, a plausible biomarker for early detection of prostate cancer." in: PLoS ONE, Vol. 7, Issue 10, pp. e46737, 2012 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Lopergolo, Tavecchio, Lisanti, Ghosh, Dohi, Faversani, Vaira, Bosari, Tanigawa, Delia, Kossenkov, Showe, Altieri: "Chk2 phosphorylation of survivin-DeltaEx3 contributes to a DNA damage-sensing checkpoint in cancer." in: Cancer research, Vol. 72, Issue 13, pp. 3251-9, 2012 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunohistochemistry

de Souza, West, Rebert, de la Motte, Drazba, Fiocchi: "Increased levels of survivin, via association with heat shock protein 90, in mucosal T cells from patients with Crohn's disease." in: Gastroenterology, Vol. 143, Issue 4, pp. 1017-26.e9, 2012 (PubMed). (Probematerial (Species): Human).

Lechler, Renkawitz, Campean, Balakrishnan, Tingart, Grifka, Schaumburger: "The antiapoptotic gene survivin is highly expressed in human chondrosarcoma and promotes drug resistance in chondrosarcoma cells in vitro." in: BMC cancer, Vol. 11, pp. 120, 2011 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence

Lechler, Schaumburger, Köck, Balakrishnan, Doukas, Prantl, Grifka: "The oncofetal gene survivin promotes cell proliferation and survival in primary human osteoblastic cells." in: Calcified tissue international, Vol. 89, Issue 3, pp. 211-20, 2011 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Western Blotting,Immunofluorescence

Lechler, Balakrishnan, Schaumburger, Grässel, Baier, Grifka, Straub, Renkawitz: "The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation in vitro." in: BMC musculoskeletal disorders, Vol. 12, pp. 150, 2011 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Knauer, Heinrich, Bier, Habtemichael, Docter, Helling, Mann, Stauber: "An otoprotective role for the apoptosis inhibitor protein survivin." in: Cell death & disease, Vol. 1, pp. e51, 2011 (PubMed).

Habtemichael, Heinrich, Knauer, Schmidtmann, Bier, Docter, Brochhausen, Helling, Brieger, Stauber, Mann: "Expression analysis suggests a potential cytoprotective role of Birc5 in the inner ear." in: Molecular and cellular neurosciences, Vol. 45, Issue 3, pp. 297-305, 2010 (PubMed).

Habtemichael, Wünsch, Bier, Tillmann, Unruhe, Frauenknecht, Heinrich, Mann, Stauber, Knauer: "Cloning and functional characterization of the guinea pig apoptosis inhibitor protein Survivin." in: Gene, Vol. 469, Issue 1-2, pp. 9-17, 2010 (PubMed).

Brocardo, Lei, Tighe, Taylor, Mok, Henderson: "Mitochondrial targeting of adenomatous polyposis coli protein is stimulated by truncating cancer mutations: regulation of Bcl-2 and implications for cell survival." in: The Journal of biological chemistry, Vol. 283, Issue 9, pp. 5950-9, 2008 (PubMed).