Survivin Antikörper (BIRC5) (Nuclear)

Details for Product anti-BIRC5 Antibody No. ABIN153037, Anbieter: Anmelden zum Anzeigen
Antigen
  • bir5a
  • survivin
  • LOC398389
  • BIRC5
  • BIR1
  • Su2
  • Survivin
  • Survivin1
  • XBIR1
  • api4
  • epr-1
  • svv1
  • xSurvivin1
  • xsurv
  • SIX
  • Su1
  • svv2
  • birc5
  • xSurvivin
  • xSurvivin2
  • AAC-11
  • Api4
  • TIAP
  • survivin40
  • baculoviral IAP repeat-containing 5a
  • survivin
  • baculoviral IAP repeat-containing 5
  • baculoviral IAP repeat-containing 5, gene 1
  • baculoviral IAP repeat containing 5
  • birc5a
  • LOC398389
  • BIRC5
  • birc5.1
  • birc5.2
  • AaeL_AAEL012446
  • CpipJ_CPIJ001663
  • CpipJ_CPIJ002689
  • CpipJ_CPIJ006694
  • Birc5
Epitop
Nuclear
65
30
26
19
17
16
15
15
12
7
7
6
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Hamster, Human
438
168
164
34
20
19
12
4
2
2
1
Wirt
Maus
300
145
16
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser Survivin Antikörper ist unkonjugiert
24
17
16
13
13
13
13
12
10
10
8
8
6
5
5
5
5
4
4
4
2
2
2
2
2
2
2
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
349
206
182
158
147
126
114
33
32
26
8
5
3
3
3
3
2
2
2
2
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Full length recombinant human Survivin [UniProt# O15392]
Klon 32-1
Isotyp IgG1 kappa
Spezifität This antibody is specific for the nuclear form of survivin.
Reinigung Protein G purified
Andere Bezeichnung Survivin (BIRC5 Antibody Abstract)
Hintergrund Gene Symbol: BIRC5
Molekulargewicht Theoretical MW: 16.5 kDa
Gen-ID 332
UniProt O15392
Forschungsgebiet Neurology, Apoptosis/Necrosis, Glia marker, Cancer
Pathways Nuclear Hormone Receptor Binding
Applikationshinweise Western Blot 1:1000, ELISA, Immunohistochemistry 20 μg/mL, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation, Immunohistochemistry-Paraffin 20 μg/mLThis Survivin (32.1) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. ELISA was reported in scientific literature. By WB, this antibody recognizes a band at ~16.5 kDa representing Survivin. *The investigator should determine the optimal working dilution for a specific application. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for Survivin Antibody IHC-FFPE sections I. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. II. Quench Endogenous Peroxidase: A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes: A. Preheat Citrate
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.9 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung -80 °C,-20 °C
Informationen zur Lagerung Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Survivin (BIRC5) (Nuclear) antibody (ABIN153037) Staining of human lung, cancer using ABIN153037.
Produkt verwendet in: Guvenc, Pavlyukov, Joshi, Kurt, Banasavadi-Siddegowda, Mao, Hong, Yamada, Kwon, Bhasin, Chettiar, Kitange, Park, Sarkaria, Li, Shakhparonov, Nakano: "Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 19, Issue 3, pp. 631-42, 2013 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunoprecipitation

Hori, Miki, Okamoto, Yazama, Konishi, Kaneko, Shimamoto, Ota, Temme, Tatsuka: "The detergent-soluble cytoplasmic pool of survivin suppresses anoikis and its expression is associated with metastatic disease of human colon cancer." in: PLoS ONE, Vol. 8, Issue 2, pp. e55710, 2013 (PubMed). Weitere Details: Western Blotting

Lechler, Renkawitz, Campean, Balakrishnan, Tingart, Grifka, Schaumburger: "The antiapoptotic gene survivin is highly expressed in human chondrosarcoma and promotes drug resistance in chondrosarcoma cells in vitro." in: BMC cancer, Vol. 11, pp. 120, 2011 (PubMed).

Lechler, Schaumburger, Köck, Balakrishnan, Doukas, Prantl, Grifka: "The oncofetal gene survivin promotes cell proliferation and survival in primary human osteoblastic cells." in: Calcified tissue international, Vol. 89, Issue 3, pp. 211-20, 2011 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Qi, Tuncel, Aoki, Tanaka, Oka, Kaneko, Okamoto, Tatsuka, Nakai, Shimamoto: "Intracellular localization of survivin determines biological behavior in colorectal cancer." in: Oncology reports, Vol. 22, Issue 3, pp. 557-62, 2009 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting,Immunohistochemistry

Lechler, Wu, Bernhardt, Campean, Gastiger, Hackenbeck, Klanke, Weidemann, Warnecke, Amann, Engehausen, Willam, Eckardt, Rödel, Wiesener: "The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney." in: The American journal of pathology, Vol. 171, Issue 5, pp. 1483-98, 2007 (PubMed). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Ding, Prieto, Zhang, Rosenthal, Smith, Skelton, Diwan: "Nuclear expression of the antiapoptotic protein survivin in malignant melanoma." in: Cancer, Vol. 106, Issue 5, pp. 1123-9, 2006 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Giodini, Kallio, Wall, Gorbsky, Tognin, Marchisio, Symons, Altieri: "Regulation of microtubule stability and mitotic progression by survivin." in: Cancer research, Vol. 62, Issue 9, pp. 2462-7, 2002 (PubMed).

Fortugno, Wall, Giodini, OConnor, Plescia, Padgett, Tognin, Marchisio, Altieri: "Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function." in: Journal of cell science, Vol. 115, Issue Pt 3, pp. 575-85, 2002 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Western Blotting,Immunoprecipitation,ELISA,Immunofluorescence

Adida, Haioun, Gaulard, Lepage, Morel, Briere, Dombret, Reyes, Diebold, Gisselbrecht, Salles, Altieri, Molina: "Prognostic significance of survivin expression in diffuse large B-cell lymphomas." in: Blood, Vol. 96, Issue 5, pp. 1921-5, 2000 (PubMed).

Grossman, McNiff, Li, Altieri: "Expression and targeting of the apoptosis inhibitor, survivin, in human melanoma." in: The Journal of investigative dermatology, Vol. 113, Issue 6, pp. 1076-81, 2000 (PubMed).

Li, Ackermann, Bennett, Rothermel, Plescia, Tognin, Villa, Marchisio, Altieri: "Pleiotropic cell-division defects and apoptosis induced by interference with survivin function." in: Nature cell biology, Vol. 1, Issue 8, pp. 461-6, 2000 (PubMed).

Allgemeine Veröffentlichungen Li, Ambrosini, Chu, Plescia, Tognin, Marchisio, Altieri: "Control of apoptosis and mitotic spindle checkpoint by survivin." in: Nature, Vol. 396, Issue 6711, pp. 580-4, 1999 (PubMed).

Ambrosini, Adida, Sirugo, Altieri: "Induction of apoptosis and inhibition of cell proliferation by survivin gene targeting." in: The Journal of biological chemistry, Vol. 273, Issue 18, pp. 11177-82, 1998 (PubMed).

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