Caspase 9 Antikörper (Caspase 9, Apoptosis-Related Cysteine Peptidase)

Details for Product anti-CASP9 Antibody No. ABIN153015, Anbieter: Anmelden zum Anzeigen
Antigen
  • CASP9
  • AI115399
  • APAF-3
  • AW493809
  • CASP-9
  • Caspase-9
  • ICE-LAP6
  • Mch6
  • Apaf3
  • Casp-9-CTD
  • Casp9_v1
  • Ice-Lap6
  • APAF3
  • MCH6
  • PPP1R56
  • caspase 9
  • caspase 9, apoptosis-related cysteine peptidase
  • caspase-9-like
  • CASP9
  • LOC100101592
  • Casp9
  • LOC100518913
Alternativen
anti-Human Caspase 9 Antikörper für ELISA
Reaktivität
Human
426
147
140
23
12
10
7
6
4
2
1
1
Wirt
Maus
420
46
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser Caspase 9 Antikörper ist unkonjugiert
11
10
7
4
4
4
4
4
4
4
4
4
4
4
4
3
3
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
402
150
132
93
74
72
53
29
22
15
13
10
6
2
2
2
1
Optionen
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Immunogen Recombinant human Caspase 9 pro-domain protein. [UniProt# P55211]
Klon LAP6 96-2-22
Isotyp IgG1 kappa
Reinigung Unpurified
Andere Bezeichnung Caspase-9 (CASP9 Antibody Abstract)
Hintergrund Gene Symbol: CASP9
Gen-ID 842
UniProt P55211
Forschungsgebiet Apoptosis/Necrosis, Cancer
Pathways MAPK Signalweg, RTK Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Endopeptidase Activity
Applikationshinweise Western Blot 1:500-1:1000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:50-1:250, Immunohistochemistry-Paraffin 1:50-1:250This Caspase 9 (LAP6 96-2-22) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where bands at ~46 kDa-48 kDa and 35 kDa are seen representing the pro and active forms of Caspase 9, respectively. In ICC/IF cytoplasmic staining can be seen in HeLa cells. Paraffin data from customer review.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Western protocol specific for Caspase 9 Antibody Procedure Guide for NB 500-209 Monoclonal anti-Caspase 9

Western Protocol

1) Run a 10-*15 % SDS polyacrylamide gel, loading ~20 µg of cell extract per lane.
2) Transfer the proteins to a membrane.
3) Block the membrane in PT-T20 [20 mM Tris-HCl, pH 7.4 / 150 mM NaCl/0.5 % Tween 20] + 5 % NFDM [non-fat dry milk], for 3 hour at room temperature (RT), shaking gently.
4) Rinse the membrane twice with PT-T20.
5) Incubate the membrane in anti-Caspase 9 [NB 500-209], diluted 1:500-1:1,000 in PT-T20 + 5 % NFDM, for 60 minutes at RT.
6) Wash the membrane for 15 minutes, 3 times, in PT-T20 at RT.
7) Incubate the membrane in anti-mouse conjugated to HRP (secondary antibody), diluted in PT-T20 + 5 % NFDM for 60 minutes at RT.
8) Wash the membrane for 15 minutes, 3 times, in PT-T20 at RT.
9) Develop membrane in chemiluminescent reagents, as instructed by kit-vendor.

*Positive control(s): Human kidney 293 cells

To Activate the Cell Extracts:
Bring the extract to a final concentration of 5 mM dATP.
Incubate the extracts at 37?C for 15-30 minutes.Immunocytochemistry/Immunofluorescence Protocol for Caspase 9 Antibody Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 30.7 mg/mL
Buffer Ascites
Buffer contains: 0.1 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung -80 °C,-20 °C
Informationen zur Lagerung Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-Caspase 9 Antikörper (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) The pro and active forms of Caspase 9 detected in active 293 cell lysate (lane 2). La...
Western Blotting (WB) image for anti-Caspase 9 Antikörper (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) Western Blot: Caspase 9 Antibody (LAP6 96-2-22) [ABIN153015] - Western blot analysis ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 9 Antikörper (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) Immunohistochemistry-Paraffin: Caspase 9 Antibody (LAP6 96-2-22) [ABIN153015] - IHC a...
Immunofluorescence (IF) image for anti-Caspase 9 Antikörper (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) Immunocytochemistry/Immunofluorescence: Caspase 9 Antibody (LAP6 96-2-22) [ABIN153015...
Produkt verwendet in: Hsuan, Chyau, Hung, Chen, Chou: "The induction of apoptosis and autophagy by Wasabia japonica extract in colon cancer." in: European journal of nutrition, Vol. 55, Issue 2, pp. 491-503, 2016 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Kim, Lee, Park, Chung, Im, Noh, Han, Moon, Jung, Ryu: "Expression of SIRT1 and apoptosis-related proteins is predictive for lymph node metastasis and disease-free survival in luminal A breast cancer." in: Virchows Archiv : an international journal of pathology, Vol. 467, Issue 5, pp. 563-70, 2015 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Martin, Fearnhead: "Apocytochrome c blocks caspase-9 activation and Bax-induced apoptosis." in: The Journal of biological chemistry, Vol. 277, Issue 52, pp. 50834-41, 2002 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Allgemeine Veröffentlichungen Kirsch, Doseff, Chau, Lim, de Souza-Pinto, Hansford, Kastan, Lazebnik, Hardwick: "Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c." in: The Journal of biological chemistry, Vol. 274, Issue 30, pp. 21155-61, 1999 (PubMed).

Fearnhead, Rodriguez, Govek, Guo, Kobayashi, Hannon, Lazebnik: "Oncogene-dependent apoptosis is mediated by caspase-9." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 23, pp. 13664-9, 1998 (PubMed).