Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1) Antikörper

Antigen: Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1)

Synonyme: ADTAA, CLAPA1, AP2-ALPHA, hip9, hypj, adtab, ap2a1, clapa2, MGC69489, AP2A1, adtaa, clapa1, ap2-alpha

Klonalität: Monoklonal (AC1-M11)

Wirt: Maus

Reaktivität: Amphibien, Rind (Kuh), Huhn, Human, Affe, Maus, Schwein, Ratte (Rattus)

Applikation: Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN152872

Produktbeschreibung

Weitere Bezeichnung: AP2A1 / ADTAA (all alpha forms)

Gen-ID: 160, 11771, 308578

Immunogen: Purified bovine brain adaptor complexes.

Format: Ascites

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: AC1-M11

Beschreibung: Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients andhormones are internalized to be recycled or down-regulated. During formation of clathrincoated membranes, clathrin co-assembles with heterotetrameric molecules known asassembly polypeptides (APs) or adaptors which form a layer of protein between theclathrin lattice and the membrane.There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrincoated vesicles at the trans-Golgi network and AP2 is associated with the endocyticclathrin coated vesicles at the plasma membrane and has been shown to specificallyinteract with Src homologous and collagen-like (Shc) and epidermal growth factor (EGF)receptor. AP2 is composed of four subunits, two separate 100 kDa gene products withsimilar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. Thereare two alpha-adaptin genes, alphaA and alphaC which have a tissue specific pattern ofexpression. Alternate Names: anti-100 kDa coated vesicle protein A antibody, anti-100 kDa coated vesicle protein Cantibody, anti-adaptin alpha A antibody, anti-Adaptin alpha B antibody, anti-Adaptorprotein complex AP 2 alpha 1 subunit antibody, anti-Adaptor protein complex AP 2alpha 2 subunit antibody, anti-Adaptor related protein complex 2 alpha 1 subunitantibody, anti-Adaptor related protein complex 2 alpha 2 subunit antibody, anti-ADTAAantibody, anti-ADTAB antibody, anti-Alpha adaptin A antibody, anti-Alpha adaptin Cantibody, anti-AP 2 complex subunit alpha 1 antibody, anti-AP 2 complex subunit alpha2 antibody, anti-AP2 alpha antibody, anti-AP2A1 antibody, anti-AP2A2 antibody,anti-CLAPA1 antibody, anti-CLAPA2 antibody, anti-Clathrin assembly protein complex 2alpha A large chain antibody, anti-Clathrin assembly protein complex 2 alpha C largechain antibody, anti-Clathrin associated/assembly/adaptor protein large alpha 1antibody, anti-Clathrin associated/assembly/adaptor protein large alpha 2 antibody,anti-HIP9 antibody, anti-Huntingtin interacting protein HYPJ antibody, anti-Huntingtininteracting protein J antibody, anti-HYPJ antibody, anti-KIAA0899 antibody, anti-Plasmamembrane adaptor HA2/AP2 adaptin alpha A subunit antibody, anti-Plasma membraneadaptor HA2/AP2 adaptin alpha C subunit antibody.

Spezifität: Detects alpha Adaptin. This antibody detects the products of both alpha-adaptin genes,alpha A and alpha C, as well as an alternatively spliced isoform of alpha A found inneurons. Species Reactivity: Cross-reacts with Human, Rat, Mouse, Cow, Pig, Chicken, Primate and Amphibians.Not yet tested in other species. Localization: Cytoplasmic.

Anwendungen

Applikationshinweise: Suggested working dilutions: immunofluorescence 1:100,Western Blot 1:100

Buffer: PBS. Preservative: 0.05% sodium azide.

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Figure 1 shows immunolocalization of alpha-adaptin in NRK cells using ABIN152872.

Publikationen

Publikationen: Wong, Brodsky: "100-kD proteins of Golgi- and trans-Golgi network-associated coated vesicles have related but distinct membrane binding properties." in: The Journal of cell biology, Vol. 117, Issue 6, pp. 1171-9, 1992 (PubMed).

Robinson: "100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies." in: The Journal of cell biology, Vol. 104, Issue 4, pp. 887-95, 1987 (PubMed).

Ball, Hunt, Robinson: "Expression and localization of alpha-adaptin isoforms." in: Journal of cell science, Vol. 108 ( Pt 8), pp. 2865-75, 1995 (PubMed).

Sorkin, Carpenter: "Interaction of activated EGF receptors with coated pit adaptins." in: Science (New York, N.Y.), Vol. 261, Issue 5121, pp. 612-5, 1993 (PubMed).

Chang, Mallet, Mostov et al.: "Adaptor self-aggregation, adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits." in: The EMBO journal, Vol. 12, Issue 5, pp. 2169-80, 1993 (PubMed).

Benmerah, Gagnon, Buegue et al.: "The tyrosine kinase substrate eps15 is constitutively associated with the plasma membrane adaptor AP-2." in: The Journal of cell biology, Vol. 131, Issue 6 Pt 2, pp. 1831-8, 1996 (PubMed).

Okabayashi, Sugimoto, Totty et al.: "Interaction of Shc with adaptor protein adaptins." in: The Journal of biological chemistry, Vol. 271, Issue 9, pp. 5265-9, 1996 (PubMed).

De Camilli, Takei: "Molecular mechanisms in synaptic vesicle endocytosis and recycling." in: Neuron, Vol. 16, Issue 3, pp. 481-6, 1996 (PubMed).

van Delft, Schumacher, Hage et al.: "Association and colocalization of Eps15 with adaptor protein-2 and clathrin." in: The Journal of cell biology, Vol. 136, Issue 4, pp. 811-21, 1997 (PubMed).

Takei, Haucke, Slepnev et al.: "Generation of coated intermediates of clathrin-mediated endocytosis on protein-free liposomes." in: Cell, Vol. 94, Issue 1, pp. 131-41, 1998 (PubMed).

Rudinskiy, Grishchuk, Vaslin et al.: "Calpain hydrolysis of alpha- and beta2-adaptins decreases clathrin-dependent endocytosis and may promote neurodegeneration." in: The Journal of biological chemistry, Vol. 284, Issue 18, pp. 12447-58, 2009 (PubMed).