phosphodiesterase 4B, CAMP-Specific (PDE4B) Antikörper

Antigen: phosphodiesterase 4B, CAMP-Specific (PDE4B)

Synonyme: DPDE4, PDE4B5, PDEIVB, MGC126529, DKFZp686F2182, MGC83972, MGC147384

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN152830

Produktbeschreibung

Weitere Bezeichnung: PDE4B

Gen-ID: 5142, 18578, 24626

Immunogen: Synthetic peptide (N terminal).

Beschreibung: PDE4B is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotidephosphodiesterase (PDE) family. Enzymes of the cAMP-dependent phosphodiesterasetype 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupledreceptor (GPCR) stimulated adenylyl cyclases. Four genes (PDE4A, B, C and D) makeup the PDE4 family. cAMP-dependent phosphodiesterase type B (PDE4B) familyconsists of four known splice variants, PDE4B1, B2, B3 and a novel 66 kDa PDE4B4protein. One or more subtype variants of PDE4B are ubiquitously present in allmammalian cells. In CNS, all four PDE4B subtype variants are expressed in varyingratios and their activity is regulated in tandem with GPCRs stimulation. Some PDE4Bvariants are also substrate for PKA-dependent phosphorylation. Peripheral tissuesexhibit differential expression of PDE4B1, B2 and B4 variants, the PDE4B4 (66kDaprotein) is the major PDE4B variant expressed in lungs, testis and heart. In testisPDE4B enzymes are expressed in somatic cells (Sertoli and Leydig cells) while PDE4Ais localized in germ cells. Alternate Names: anti-cAMP specific 3', 5', cyclic phosphodiesterase 4B antibody,anti-cAMP-specific 3',5',-cyclic Phosphodiesterase 4B antibody,anti-DKFZp686F2182 antibody, anti-DPDE 4 antibody, anti-DPDE4 antibody,anti-Dunce like phosphodiesterase E4 antibody, anti-MGC126529 antibody,anti-OTTHUMP00000046896 antibody, anti-PDE 32 antibody, anti-PDE 4B antibody,anti-PDE IVB antibody, anti-PDE32 antibody, anti-PDE4B antibody, anti-PDEIVBantibody, anti-Phosphodiesterase 4B antibody, anti-Phosphodiesterase E4 duncehomolog Drosophila antibody, anti-Phosphodiesterase-4B cAMP-specific (dunce(Drosophila)-homolog) antibody, anti-Phosphodiesterase-4B cAMP-specific antibody.

Spezifität: This antibody detects all PDE4B proteins, including PDE4B1, PDE4B2, PDE4B3 and a66 kDa PDE4B variant. It does not cross-react with PDE4A, PDE4C or PDE4D. Species Reactivity: Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.

Anwendungen

Applikationshinweise: ICC: Use at a dilution of 1/300. IF: Use at a dilution of 1/200. IP: Use at a dilution of1/350. WB: Use at a dilution of 1/1500. Detects bands of 107 kDa (PDE4B1), 78 kDa(PDE4B2), 100 kDa (PDE4B3) and 66 kDa (PDE4B4).

Konzentration: 0.65 mg/ml

Buffer: Preservative: NoneConstituents: 20% Glycerol, PBS. Preservative: NoneConstituents: 20% Glycerol, PBS.

Lagerung: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Forschungsgebiet: Signaltransduktion, Enzyme, Metabolismus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-phosphodiesterase 4B, CAMP-Specific (PDE4B) antibody