ATPase, Na+/K+ Transporting, alpha 3 Polypeptide (ATP1A3) Antikörper

Antigen: ATPase, Na+/K+ Transporting, alpha 3 Polypeptide (ATP1A3)

Synonyme: RDP, DYT12, MGC13276, MGC3285, MGC51750, Atpa-1, BC010319, MGC38419, Nkaa1b, ATP1A1

Klonalität: Monoklonal (XVIF9-G10)

Wirt: Maus

Reaktivität: Hund, Meerschweinchen, Human, Affe, Kaninchen, Ratte (Rattus), Schaf

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN152699

Produktbeschreibung

Weitere Bezeichnung: ATP1A3

Gen-ID: 478, 24213

Immunogen: Canine cardiac microsomes.

Format: Ascites

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: XVIF9-G10

Beschreibung: The sodium/potassium ATPase is an integral membrane enzyme found in all cells ofhigher organisms and is responsible for the ATP-dependent transport of sodium andpotassium across the cell membrane. This membrane-bound enzyme is related to anumber of other ATPases including sarcoplasmic and endoplasmic reticulum calciumATPase (SERCA) and plasma membrane calcium ATPase (PMCA). Thesodium/potassium ATPase consists of a large, multipass, transmembrane catalyticsubunit, termed the alpha subunit, and an associated smaller glycoprotein, termed thebeta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1,alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded bytwo multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific anddevelopmental patterns of expression. The alpha 1 and beta mRNAs are present in allcell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restrictedpattern of cell-specific expression. The alpha-3 subunit has been found in neuronal andto skeletal and cardiac muscle, lung and stomach tissues.

Spezifität: Detects sodium / potassium ATPase. This antibody is specific for the alpha-3 subunit. Species Reactivity: Cross-reacts with Human, Rabbit, Rat, Dog, Sheep, Guinea pig and Primate. Not yettested in other species.

Anwendungen

Applikationshinweise: This antibody can be used in Western Blot, Immunofluorescence, andImmunohistochemistry (frozen sections). By Western blot, this antibody detects an~110 kDa protein representing the alpha-3 subunit of the sodium / potassium ATPasefrom canine skeletal muscle extract. Immunohistochemical staining of sodium /potassium ATPase in rat retina yields a pattern consistent with plasma membranelocalization.Recommended Starting Dilutions*:IHC: 3 ug/ml WB: 1 ug/mlIF: 5 ug/ml*Optimal dilutions should be determined by the end user

Konzentration: 1 mg/ml

Buffer: PBS containing 0.05% sodium azide. Preservative: Contains 0.05% sodium azide.

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Lingrel, Orlowski, Shull et al.: "Molecular genetics of Na,K-ATPase." in: Progress in nucleic acid research and molecular biology, Vol. 38, pp. 37-89, 1990 (PubMed).

Sweadner: "Isozymes of the Na+/K+-ATPase." in: Biochimica et biophysica acta, Vol. 988, Issue 2, pp. 185-220, 1989 (PubMed).

Arystarkhova, Sweadner: "Isoform-specific monoclonal antibodies to Na,K-ATPase alpha subunits. Evidence for a tissue-specific post-translational modification of the alpha subunit." in: The Journal of biological chemistry, Vol. 271, Issue 38, pp. 23407-17, 1996 (PubMed).

Hou, Matherly: "Oligomeric structure of the human reduced folate carrier: identification of homo-oligomers and dominant-negative effects on carrier expression and function." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 3285-93, 2009 (PubMed).