O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) Antikörper

Antigen: O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT)

Synonyme: HRNT1, FLJ23071, MGC22921, O-GLCNAC, MGC80426, MGC69550

Klonalität: Monoklonal (RL2)

Wirt: Maus

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN152683

Produktbeschreibung

Weitere Bezeichnung: O-GlcNAc

Gen-ID: 8473, 108155, 26295

Immunogen: Pore complex-lamina fraction purified from rat liver nuclear envelopes.

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: RL2

Beschreibung: Diffusion of metabolites and small non-nuclear molecules as well as active, mediatedimport of protein and export of protein and RNA through the nuclear envelope occursthrough nuclear pore complexes or NPC's. NPC's contain up to 100 differentpolypeptides which have a combined mass of about 125 megadaltons. The channelavailable for passive transport through the NPC is about 9-10 nm in diameter whilecarrier mediated changes in the NPC result in a ~25 nm channel used for larger, activelytransported molecules. Of the 100 polypeptides, at least 8 of these are O-linkedN-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linkedglycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeatsdispersed throughout part of their sequence. Studies indicate that the NPC O-linkedglycoproteins have a direct role in nuclear protein import. Alternate Names: anti-O-GlcNAc antibody.

Spezifität: Detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linkedglycoproteins from human, mouse, and rat tissues. Species Reactivity: Human, Mouse, Rat. Localization: Cytoplasmic and Nuclear.

Anwendungen

Applikationshinweise: Suggested working dilutions: immunofluorescence 1:100,Western Blot 1:1,000,Immunohistochemistry-Paraffin 1:200

Buffer: PBS. Preservative: 0.05% sodium azide.

Lagerung: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar