Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Antikörper

Antigen: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

Synonyme: G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17

Klonalität: Monoklonal (2D4A7)

Wirt: Maus

Reaktivität: Human, Maus

Applikation: Enzyme Immunoassay (EIA), Western Blot (WB)

Produktnummer: ABIN152657

Produktbeschreibung

Weitere Bezeichnung: GAPDH

Gen-ID: 2597, 14433

Swiss-Prot: P04406

Immunogen: Ni-NTA purified recombinant human GAPDH expressed in E. Coli strain BL21 (DE3).

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 2D4A7

Beschreibung: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the keyenzymes involved in glycolysis. It catalyzes an important energy-yielding step incarbohydrate metabolism, the reversible oxidative phosphorylation ofglyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamideadenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggestthat mammalian GAPDH is also involved in a great number of intracellular procesessuch as membrane fusion, microtubule bundling, phosphotransferase activity, nuclearRNA export, DNA replication, and DNA repair. During the last decade a lot of findingsappeared concerning the role of GAPDH in different pathologies including prostatecancer progression, programmed neuronal cell death, age- related neuronal diseases,such as Alzheimer's and Huntington's disease. Alternate Names: anti-GAPDH antibody, anti-glyceraldehyde-3-phosphate dehydrogenase antibody,anti-HGNC:4141 antibody, anti-G3PD antibody, anti-GAPD antibody, anti-MGC88685antibody.

Spezifität: Species Reactivity: ABIN152657 reacts with GAPDH from human and mouse. Since GAPDH is one of themost conserved proteins known, it is likely that the antibody is effective on other speciesalso.

Synonyme: G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17

Anwendungen

Applikationshinweise: ABIN152657 can be used in Western blot and ELISA. Other applications have not beentested. Suggested working dilutions: *Western Blot - 1:20,000+ (ECL) ****Tested on HeLa WCE at 1:20,000

Konzentration: 0.68 mg/ml

Buffer: Tris-glycine and 150 mM NaCl, containing 0.05% sodium azide. Preservative: 0.05% sodium azide

Lagerung: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Forschungsgebiet: Krebs, Metabolismus, Alzheimer-Krankheit (Morbus Alzheimer), Enzyme

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Detection of GAPDH in mouse liver using ABIN152657.

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (Image 2)

Publikationen

Publikationen: Allen, Trach, Hoch: "Identification of the 37-kDa protein displaying a variable interaction with the erythroid cell membrane as glyceraldehyde-3-phosphate dehydrogenase." in: The Journal of biological chemistry, Vol. 262, Issue 2, pp. 649-53, 1987 (PubMed).