Quinone Reductase 1 (NQO1) Antikörper

Antigen: Quinone Reductase 1 (NQO1)

Synonyme: DTD, QR1, DHQU, DIA4, NMOR1, NMORI, Dtd, Ox1, Qr1, Dia4, Nmo1, Ox-1, Nmo-1, Nmor1, AV001255, MGC93075

Klonalität: Monoklonal (AI80)

Wirt: Maus

Reaktivität: Hund, Human, Affe

Applikation: Immunfluoreszenz (IF), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN152344

Produktbeschreibung

Weitere Bezeichnung: NQO1

Gen-ID: 1728, 18104, 24314

Swiss-Prot: P15559

Immunogen: Full length recombinant NQO1 (from human lung).

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: AI80

Beschreibung: NQO1 is a 2-electron reductase that detoxifies quinones derived from the oxidation ofphenolic metabolites of benzene. Individuals homozygous for the T/T form of the609C-T polymorphism have an increased risk of benzene hematotoxicity. Alternate Names: anti-DHQU antibody, anti-DIA4 antibody, anti-Diaphorase (NADH/NADPH) (cytochromeb-5 reductase) antibody, anti-Diaphorase (NADH/NADPH) antibody, anti-Diaphorase 4antibody, anti-DTD antibody, anti-NAD(P)H dehydrogenase quinone 1 antibody,anti-NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody, anti-NMOR1antibody, anti-NMORI antibody, anti-QR1 antibody, anti-NQO-1 antibody, anti-NADPHquinone oxidoreductase-1 antibody.

Spezifität: This antibody is specific for NQO1. It does not cross-react with NQO2. Species Reactivity: Cross-reacts with human and rat. Cross-reacts with monkey and dog inimmunohistochemistry. Cross-reactivity with mouse is extremely poor. Not yet tested inother species. Localization: Cytoplasmic (primarily), nuclear (faintly).

Anwendungen

Applikationshinweise: Suggested working dilutions: Western Blot 1:1,000-1:10,000 (ECL)

Konzentration: 1.0 mg/ml

Buffer: Tris-glycine and 150mM NaCl. Preservative: Sodium Azide

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Forschungsgebiet: Signaltransduktion, Metabolismus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Detection of NQO1 in A549 lysates using ABIN152344. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.

Publikationen

Publikationen: Siegel, Franklin, Ross: "Immunohistochemical detection of NAD(P)H:quinone oxidoreductase in human lung and lung tumors." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 4, Issue 9, pp. 2065-70, 1998 (PubMed).

Slitt, Cherrington, Dieter et al.: "trans-Stilbene oxide induces expression of genes involved in metabolism and transport in mouse liver via CAR and Nrf2 transcription factors." in: Molecular pharmacology, Vol. 69, Issue 5, pp. 1554-63, 2006 (PubMed).

Lee, Bhora, Sun et al.: "Dietary flaxseed enhances antioxidant defenses and is protective in a mouse model of lung ischemia-reperfusion injury." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 294, Issue 2, pp. L255-65, 2008 (PubMed).

Cho, Imani, Miller-DeGraff et al.: "Antiviral activity of Nrf2 in a murine model of respiratory syncytial virus disease." in: American journal of respiratory and critical care medicine, Vol. 179, Issue 2, pp. 138-50, 2009 (PubMed).

Salama, Kamel, Diaz-Arrastia et al.: "Effect of tumor necrosis factor-alpha on estrogen metabolism and endometrial cells: potential physiological and pathological relevance." in: The Journal of clinical endocrinology and metabolism, Vol. 94, Issue 1, pp. 285-93, 2009 (PubMed).