MYCN Antikörper (Mycn)

Details for Product anti-MYCN Antibody No. ABIN152254, Anbieter: Anmelden zum Anzeigen
Antigen
  • MODED
  • MYCNOT
  • N-myc
  • NMYC
  • ODED
  • bHLHe37
  • Nmuc1
  • Nmyc
  • Nmyc-1
  • Nmyc1
  • c-nmyc
  • olfactory receptor, family 8, subfamily S, member 1-like
  • v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog
  • v-myc myelocytomatosis viral related oncogene, neuroblastoma derived
  • v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
  • LOC515887
  • MYCN
  • Mycn
Reaktivität
Human, Maus
90
55
30
3
2
2
2
2
1
1
1
Wirt
Maus
58
34
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser MYCN Antikörper ist unkonjugiert
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Chromatin Immunoprecipitation (ChIP), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
69
20
17
16
15
13
5
4
3
2
2
2
2
1
1
1
Optionen
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Immunogen A synthetic peptide (SPYVESEDAPPQKC) conjugated to KLH, corresponding to amino acids 327-339 of human n-Myc. [UniProt# P04198]
Klon NMYC-1
Isotyp IgG2a kappa
Spezifität This does not react with c-Myc or L-Myc.
Reinigung Protein A purified
Andere Bezeichnung N-Myc (MYCN Antibody Abstract)
Hintergrund Gene Symbol: MYCN
Gen-ID 4613
UniProt P04198
Applikationshinweise Western Blot 1:500-1:1000, Chromatin Immunoprecipitation 1:10-1:500, Flow Cytometry, Immunocytochemistry/Immunofluorescence 1:50-1:100, Immunoprecipitation 1:10-1:100, Gel Super Shift Assays 1:1-1:100, CyTOF-readyThis n-Myc (NMYC-1) antibody is useful for Immunocytochemistry/ Immunofluorescence, Immunoprecipitation and Western Blot, where a band can been seen at approximately 50-65 kDa. This antibody has also been used for Chromatin Immunoprecipitation (PMID: 21068393) and Gel Super Shift Assays (PMID: 21068393). Use in FLOW cytometry reported in scientific literature (PMID 27539729).
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Western Blot Protocol for NB200-109: n-Myc Antibody (NMYC-1)Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Bilder des Herstellers
Immunofluorescence (IF) image for anti-MYCN Antikörper (Mycn) (ABIN152254) Immunocytochemistry/ Immunofluorescence: n-Myc Antibody (NMYC-1) [ABIN152254] - The n...
Western Blotting (WB) image for anti-MYCN Antikörper (Mycn) (ABIN152254) Western Blot: n-Myc Antibody (NMYC-1) [ABIN152254] - Western blot analysis of n-Myc e...
Western Blotting (WB) image for anti-MYCN Antikörper (Mycn) (ABIN152254) Western Blot: n-Myc Antibody (NMYC-1) [ABIN152254] - Western blot analysis of n-Myc e...
Produkt verwendet in: Murakami-Tonami, Ikeda, Yamagishi, Inayoshi, Inagaki, Kishida, Komata, Jan Koster, Takeuchi, Kondo, Maeda, Sekido, Murakami, Kadomatsu: "SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells." in: Scientific reports, Vol. 6, pp. 31615, 2016 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting,Chromatin Immunoprecipitation,Flow Cytometry

Murakami-Tonami, Kishida, Takeuchi, Katou, Maris, Ichikawa, Kondo, Sekido, Shirahige, Murakami, Kadomatsu: "Inactivation of SMC2 shows a synergistic lethal response in MYCN-amplified neuroblastoma cells." in: Cell cycle (Georgetown, Tex.), Vol. 13, Issue 7, pp. 1115-31, 2014 (PubMed).

Tasseva, Cole, Vance: "N-Myc and SP regulate phosphatidylserine synthase-1 expression in brain and glial cells." in: The Journal of biological chemistry, Vol. 286, Issue 2, pp. 1061-73, 2011 (PubMed).