Histone Core Antikörper

Antigen: Histone Core

Klonalität: Polyklonal

Wirt: Schaf

Reaktivität: Rind (Kuh)

Applikation: Enzyme Immunoassay (EIA), Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN152164

Produktbeschreibung

Immunogen: Intact calf H-3 complexed to rRNA.

Beschreibung: In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. Theyconsist of two major components in equal amounts, DNA and basic proteins calledhistones. Histones are believed to be regularly arranged in the deep grove of the DNAhelix. The recurring positive charges of the histones form electrostatic associations withthe negatively charged phosphate groups of DNA making the DNA more stable andflexible. This allows for the supercoiling of the chromatin fibers.The histone core protein is an octamer consisting of 2 subunits of each of histone 2A,2B, 3 and 4. In conjunction with the linker histone H1 and the nonhistone chromosomalproteins, it is responsible for the binding and compacting of DNA into chromatin. Thesequences of the core histones are highly conserved from species to species.

Spezifität: This antibody is specific for Histone-Core. Species Reactivity: Cross-reacts with Cow. Not yet tested in other species.

Anwendungen

Applikationshinweise: Immunofluorescence. Immunoprecipitaion. Western Blot. Enzyme-linkedimmunosorbent assay

Buffer: Phosphate buffered saline

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, DNS/RNS, Transkriptionsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar