E1A Binding Protein P300 (EP300) Antikörper

Antigen: E1A Binding Protein P300 (EP300)

Synonyme: p300, KAT3B, EP300

Klonalität: Monoklonal (RW128)

Wirt: Maus

Reaktivität: Human, Nerz, Affe, Maus, Ratte (Rattus)

Applikation: Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN151948

Produktbeschreibung

Weitere Bezeichnung: EP300 / P300

Gen-ID: 2033, 328572, 170915

Immunogen: Fusion protein containing residues 1572-2371 of human p300.

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: RW128

Beschreibung: P300 is a relatively stable, ubiquitously expressed, nuclear phosphoprotein which isconserved among a variety of mammalian species. It is actively synthesized andphosphorylated in both quiescent and proliferating cells.Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) andp300 are closely related transcriptional coactivators and have been shown to directlyinteract with many different DNA-binding transcription factors including nuclear hormonereceptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Both CBP andp300 have been shown to display histone acetyltransferase (HAT) activity, capable ofacetylating all four core histone particles in nucleosomes. As a result of HAT activity, ithas been suggested CBP and p300 may play a direct role in activating chromatin fortranscription. Alternate Names: anti-CREBBP/EP300 inhibitory protein 1 antibody, anti-Cyclic AMP responsive enhancerbinding protein antibody, anti-EP300: E1A binding protein p300 antibody, anti-RB andP300 binding protein EID 1 antibody, anti-Retinoblastoma protein associated proteinantibody.

Spezifität: This antibody is specific for p300 protein. This antibody recognizes residues1868-1921. Species Reactivity: Human, mouse, rat, monkey, and mink protein. Localization: Nuclear.

Anwendungen

Applikationshinweise: Suggested working dilutions: Western Blot 1:250-1:500

Konzentration: 1 mg/ml

Buffer: PBS [pH7.4]

Lagerung: Aliquot and store at -20C or below. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Detection of p300 in a HeLa nuclear extract using ABIN151948 (1:250). ECL: 15 second exposure.

Publikationen

Publikationen: Eckner, Ewen, Newsome et al.: "Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor." in: Genes & development, Vol. 8, Issue 8, pp. 869-84, 1994 (PubMed).

Eckner, Ludlow, Lill et al.: "Association of p300 and CBP with simian virus 40 large T antigen." in: Molecular and cellular biology, Vol. 16, Issue 7, pp. 3454-64, 1996 (PubMed).

Eckner, Yao, Oldread et al.: "Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation." in: Genes & development, Vol. 10, Issue 19, pp. 2478-90, 1996 (PubMed).