PCNA Antikörper

Antigen: PCNA

Klonalität: Monoklonal (339)

Wirt: Maus

Reaktivität: Human

Applikation: Enzyme Immunoassay (EIA), Western Blot (WB)

Produktnummer: ABIN151925

Produktbeschreibung

Gen-ID: 5111

Immunogen: Recombinant PCNA interdomain connector loop protein. This immunogen is comprisedof residues 107-196 of human PCNA. (The C-terminal portion of this antigen does notextend beyond the true IDCL of PCNA, and as such, also includes part of the PCNAD2E2 loop and the protein in between.).

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: 339

Beschreibung: PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found inthe nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer andhelps increase the processivity of leading strand synthesis during DNA replication. Inresponse to DNA damage, PCNA is ubiquitinated and is involved in the RAD6dependent DNA repair pathway. Two transcript variants encoding the same protein havebeen found for PCNA. Pseudogenes of this gene have been described on chromosome4 and on the X chromosome. Alternate Names: anti-Cyclin antibody, anti-DNA polymerase delta au xiliary protein antibody,anti-HGCN8729 antibody, anti-MGC8367 antibody, anti-polymerase delta accessoryprotein antibody, anti-Proliferating Cell Nuclear Antigen antibody.

Spezifität: This antibody reacts with the interdomain connector loop of human PCNA, other notdetermined. Species Reactivity: Cross-reacts with Human. Not yet tested in other species. Localization: Nuclear.

Anwendungen

Applikationshinweise: Western Blot 1/500 - 1/1000

Konzentration: 1 mg/ml

Buffer: Phosphate-buffered saline, pH 7.2. Preservative: No preservatives.

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Forschungsgebiet: Krebs, Zellzyklus, Chromatin-Bindungsproteine, DNS/RNS, Proliferationsmarker

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-PCNA antibody

Publikationen

Publikationen: Warbrick, Lane, Glover et al.: "Homologous regions of Fen1 and p21Cip1 compete for binding to the same site on PCNA: a potential mechanism to co-ordinate DNA replication and repair." in: Oncogene, Vol. 14, Issue 19, pp. 2313-21, 1997 (PubMed).

Warbrick: "PCNA binding through a conserved motif." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 20, Issue 3, pp. 195-9, 1998 (PubMed).

Levin, McKenna, Motycka et al.: "Interaction between PCNA and DNA ligase I is critical for joining of Okazaki fragments and long-patch base-excision repair." in: Current biology : CB, Vol. 10, Issue 15, pp. 919-22, 2000 (PubMed).

Malkas, Herbert, Abdel-Aziz et al.: "A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 51, pp. 19472-7, 2006 (PubMed).