RECQL2 (ARABIDOPSIS RECQ HELICASE L2); 3'-5' DNA Helicase/ ATP-Dependent Helicase/ Four-Way Junction Helicase/ Protein Binding (RECQL2) Antikörper

Antigen: RECQL2 (ARABIDOPSIS RECQ HELICASE L2); 3'-5' DNA Helicase/ ATP-Dependent Helicase/ Four-Way Junction Helicase/ Protein Binding (RECQL2)

Synonyme: RECQ3, RECQL2, RECQL3, DKFZp686C2056, ARABIDOPSIS RECQ HELICASE L2, ARABIDOPSIS THALIANA RECQ 2, ATRECQ2, T19E23.16, T19E23_16, xBLM, recq2, recql2, recql3

Klonalität: Monoklonal (WRN)

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN151918

Produktbeschreibung

Weitere Bezeichnung: WRN

Gen-ID: 7486

Swiss-Prot: Q14191

Immunogen: The epitope recognized by this antibody maps to a region between residues 400 and450 of human Werner Syndrome Helicase using the numbering given in SwissProt entryQ14191 (GeneID 7486).

Klon: WRN

Beschreibung: This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His)subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects ofDNA metabolism, including transcription, replication, recombination, and repair. Thisprotein contains a nuclear localization signal in the C-terminus and shows apredominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicaseactivity, and is also a 3' to 5' exonuclease. Based on interactions between this proteinand Ku70/80 heterodimer in DNA end processing, this protein may be involved in therepair of double strand DNA breaks. Defects in this gene are the cause of WernerSyndrome, an autosomal recessive disorder characterized by premature aging. Alternate Names: anti-HGNC 12791 antibody, anti-RECQ3 antibody, anti-RECQL2 antibody, anti-RECQL3antibody, anti-werner syndrome helicase antibody, anti-werner syndrome proteinantibody, anti-WRN antibody.

Spezifität: This antibody is specific for human WRN. Species Reactivity: Human protein. Reactivity with Mouse WRN is unlikely owing to divergence between theMouse sequence and the peptide used for this antibody production.

Anwendungen

Applikationshinweise: Suggested working dilutions: immunoprecipitation 2 to 5 ug/mg lysate,Western Blot 1:5,000 to 1:25,000(ECL)

Konzentration: 1 mg/ml

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8. Preservative: 0.09% Sodium Azide.

Lagerung: Store at 4C. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Detection of WRN in 293T whole cell lysate using ABIN151918.

Detection of human WRN by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (WCL, 100 mcg for WB, one 10cm plate for IP/WB) from 293T cells. Antibodies: Affinity purified rabbit anti-WRN antibody (Cat. No. ABIN151918) used at 0.1 mcg/ml for WB. WRN was immunoprecipitated with ABIN151918 or rabbit anti-WRN antibody (Cat. No. ABIN151919) each used at 5 mcg/10cm plate (+/- respective blocking peptide). Detection: Chemiluminescence with an exposure time of less than 10 seconds.

Publikationen

Publikationen: Harrigan, Piotrowski, Di Noto et al.: "Metal-catalyzed oxidation of the Werner syndrome protein causes loss of catalytic activities and impaired protein-protein interactions." in: The Journal of biological chemistry, Vol. 282, Issue 50, pp. 36403-11, 2007 (PubMed).

Nyunoya, Monick, Klingelhutz et al.: "Cigarette smoke induces cellular senescence via Werner's syndrome protein down-regulation." in: American journal of respiratory and critical care medicine, Vol. 179, Issue 4, pp. 279-87, 2009 (PubMed).