Nuclear Receptor Coactivator 1 (NCOA1) Antikörper

Antigen: Nuclear Receptor Coactivator 1 (NCOA1)

Synonyme: SRC1, KAT13A, RIP160, F-SRC-1, bHLHe42, bHLHe74, MGC129719, MGC129720, NCOA1, si:dkey-5g7.6, si:dkey-34f16.6, SRA, SRAP, STRAA1, pp7684, MGC87674, Sra, Srap, Straa1, Strra1, AA959952, zgc:86613, SRA1, MGC128615, DKFZp459C083

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN151779

Produktbeschreibung

Weitere Bezeichnung: NCOA1

Gen-ID: 8648

Swiss-Prot: NP_0065311

Immunogen: A 37 amino acid synthetic peptide, which represented a portion of human nuclearreceptor coactivator 1 encoded within exon 23 (LocusLink ID 8648).

Beschreibung: Steroid and thyroid hormones and retinoic acid regulate a complex array of geneexpression activity via intracellular receptor transcription factors belonging to the liganddependent nuclear receptor superfamily. Adding to the complexity of function of thesetranscription factors are associated proteins known as coactivators and corepressorswhich, as their names suggest, enhance or depress transcriptional activity of the nuclearreceptor with which they associate. One such coactivator is Steroid ReceptorCoactivator-1 (SRC 1), a 110 kDa protein.SRC 1 has been shown to interact with and stimulate the transcriptional activity ofestrogen, progesterone, retinoic acid, thyroid and glucocorticoid receptors, as well asretinoic X receptor (RXR) in a ligand dependent manner. The amino terminal region ofSRC 1 contains a PAS-A-basic helix-loop-helix homology domain which has previouslybeen shown to be dimerization domains in other nuclear transcription factors includingthe aryl hydrocarbon (Ah) receptor and its heterodimerization partner, ARNT. Thecarboxy-terminal region of SRC 1 has been shown to possess histone acetyltransferaseactivity specific primarily for histones H3 & H4.

Spezifität: ABIN151779 is specific for human SRC1 protein. Species Reactivity: ABIN151779 reacts with human. Other species have not been tested. Mouse sharecomplete residue homology with the peptide used as the immunogen for this antibody.

Synonyme: SRC1, KAT13A, RIP160, F-SRC-1, bHLHe42, bHLHe74, MGC129719, MGC129720, NCOA1, si:dkey-5g7.6, si:dkey-34f16.6, SRA, SRAP, STRAA1, pp7684, MGC87674, Sra, Srap, Straa1, Strra1, AA959952, zgc:86613, SRA1, MGC128615, DKFZp459C083

Anwendungen

Applikationshinweise: ABIN151779 may be used for Western blot and immunoprecipitation (HeLa). Itimmunoprecipitates nuclear extracts better than cytosolic extracts.Suggested working dilutions: *Western Blot - 1:5,000, Immunocytochemistry - Not DoneImmunoprecipitation - 1-4 ug/mg lysate

Konzentration: 1 mg/ml

Buffer: PBS, 1 mg/ml BSA

Lagerung: Store at 4C. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Heemers, Regan, Schmidt et al.: "Androgen modulation of coregulator expression in prostate cancer cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 23, Issue 4, pp. 572-83, 2009 (PubMed).