ATM Antikörper (Ataxia Telangiectasia Mutated) (pSer1981)

Details for Product anti-ATM Antibody No. ABIN151772, Anbieter: Anmelden zum Anzeigen
Antigen
  • ATM
  • Atm
  • CG6535
  • Dmel\\CG6535
  • Tefu
  • atm
  • atm/tefu
  • dATM
  • tef
  • Xatm
  • at1
  • atdc
  • tel1
  • telo1
  • AT1
  • ATA
  • ATC
  • ATD
  • ATDC
  • ATE
  • TEL1
  • TELO1
  • AI256621
  • C030026E19Rik
  • telomere fusion
  • ataxia telangiectasia mutated
  • ataxia telangiectasia mutated (atm)
  • ataxia telangiectasia mutated homolog (human)
  • tefu
  • atm
  • ATM
  • EDI_100660
  • CpipJ_CPIJ001772
  • BDBG_08252
  • PAAG_02532
  • MCYG_05088
  • VDBG_06833
  • ACLA_015700
  • AaeL_AAEL014900
  • MGYG_07634
  • PGTG_14279
  • Atm
  • LOC100349299
Alternativen
anti-Ratte (Rattus) ATM Antikörper für Chromatin Immunoprecipitation
Epitop
pSer1981
87
40
17
16
16
15
8
8
6
6
6
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
352
156
76
27
8
1
1
1
1
Wirt
Maus
229
96
17
9
3
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser ATM Antikörper ist unkonjugiert
12
11
10
9
6
6
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
213
124
109
87
85
70
58
45
27
21
13
6
4
4
2
2
2
1
1
Optionen
Hersteller
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Immunogen Synthetic peptide made to a region surrounding the phosphorylated Serine 1981 of human ATM. [UniProt# Q13315]
Klon 10H11-E12
Isotyp IgG1 kappa
Keine Kreuzreaktivität Hund
Reinigung Protein G purified
Andere Bezeichnung ATM (ATM Antibody Abstract)
Hintergrund Gene Symbol: ATM
Molekulargewicht Theoretical MW: 370 kDa
Gen-ID 472
UniProt Q13315
Forschungsgebiet DNA/RNA, Cancer
Pathways p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus
Applikationshinweise Western Blot 1:1000, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:1000, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:200This ATM [p Ser1981] (10H11.E12) antibody useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band at ~370 kDa can be seen. In IHC-P, staining was observed in the nucleus and cytoplasm of mouse spleen. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for ATM Kinase Antibody Western Blot Procedure1) Resolve protein samples on a 6 % SDS-PAGE gel at 185V for ~1.5 hours.
2) Transfer to PVDF membranes at 25V for ~1.5 hours.
) Block the membrane with TBST+BSA and goat serum for 1 hour at RT.
) Dilute primary antibody to 1:1,000 in blocking buffer.
) Incubate membrane overnight at 4 degrees Celcius in diluted anti-ATM-kinase.
) Wash 3 times ten minutes on a shaker.
) Incubate membranes with HRP conjugated anti-mouse IgG for 1 hour (RT), diluted in blocking buffer.
) Wash 3 times ten minutes on a shaker.
) Add ECL reagent, as per kit directions, and expose for 1-5 seconds.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.97 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung -80 °C,-20 °C
Informationen zur Lagerung Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-ATM Antikörper (Ataxia Telangiectasia Mutated) (pSer1981) (ABIN151772) Western Blot analysis of ATM-kinase, using ABIN151772. Sample: Irradiated or peroxida...
Immunohistochemistry (IHC) image for anti-ATM Antikörper (Ataxia Telangiectasia Mutated) (pSer1981) (ABIN151772) Immunohistochemistry: ATM [p Ser1981] Antibody (10H11.E12) [ABIN151772] - ATM antibod...
Produkt verwendet in: Liu, Liu, Gao, Reske, Li, Wu, Yang, Chen, Luo, Xiao: "A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation." in: Cancer letters, Vol. 391, pp. 152-161, 2017 (PubMed). Von den Autoren verwendete Methode: Immunofluorescence (fixed cells) (IF/ICC) (Probematerial (Species): Human).

Wu, Wang, Chen, Chao, Chang, Chen, Hung: "Early Passage Mesenchymal Stem Cells Display Decreased Radiosensitivity and Increased DNA Repair Activity." in: Stem cells translational medicine, Vol. 6, Issue 6, pp. 1504-1514, 2017 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Takai, Jenkinson, Kabir, Babul-Hirji, Najm-Tehrani, Chitayat, Crow, de Lange: "A POT1 mutation implicates defective telomere end fill-in and telomere truncations in Coats plus." in: Genes & development, Vol. 30, Issue 7, pp. 812-26, 2016 (PubMed). (Probematerial (Species): Human).

Banerjee, Banerjee, Mandal: "Enhanced chemotherapeutic efficacy of apigenin liposomes in colorectal cancer based on flavone-membrane interactions." in: Journal of colloid and interface science, Vol. 491, pp. 98-110, 2016 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (IHC) (Probematerial (Species): Human).

Yang, Shen, Oishi, Matteson, Tian, Goronzy, Weyand: "Restoring oxidant signaling suppresses proarthritogenic T cell effector functions in rheumatoid arthritis." in: Science translational medicine, Vol. 8, Issue 331, pp. 331ra38, 2016 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Liedtke, Biebernick, Radke, Stapelkamp, Coenen, Zaehres, Fritz, Kogler: "DNA damage response in neonatal and adult stromal cells compared with induced pluripotent stem cells." in: Stem cells translational medicine, Vol. 4, Issue 6, pp. 576-89, 2015 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Caserta, Egriboz, Wang, Martin, Koivisto, Pecót, Kladney, Shen, Shim, Pham, Karikomi, Mauntel, Majumder, Cuitino, Tang, Srivastava, Yu, Wallace, Mo, Park, Fernandez, Pilarski, La Perle, Rosol et al.: "Noncatalytic PTEN missense mutation predisposes to organ-selective cancer development in vivo. ..." in: Genes & development, Vol. 29, Issue 16, pp. 1707-20, 2015 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Sayin, Ibrahim, Larsson, Nilsson, Lindahl, Bergo: "Antioxidants accelerate lung cancer progression in mice." in: Science translational medicine, Vol. 6, Issue 221, pp. 221ra15, 2014 (PubMed). Weitere Details: Western Blotting

Park, Jeon, Kim: "Selenoprotein W promotes cell cycle recovery from G2 arrest through the activation of CDC25B." in: Biochimica et biophysica acta, Vol. 1823, Issue 12, pp. 2217-26, 2012 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Someya, Sakata, Matsumoto, Tauchi, Kai, Hareyama, Fukushima: "Effects of depletion of dihydropyrimidine dehydrogenase on focus formation and RPA phosphorylation." in: Journal of radiation research, Vol. 53, Issue 2, pp. 250-6, 2012 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Guerra, Albihn, Tronnersjö, Yan, Guidi, Stenerlöw, Sterzenbach, Josenhans, Fox, Schauer, Thelestam, Larsson, Henriksson, Frisan: "Myc is required for activation of the ATM-dependent checkpoints in response to DNA damage." in: PLoS ONE, Vol. 5, Issue 1, pp. e8924, 2010 (PubMed). Weitere Details: Western Blotting

Gastaldello, Hildebrand, Faridani, Callegari, Palmkvist, Di Guglielmo, Masucci: "A deneddylase encoded by Epstein-Barr virus promotes viral DNA replication by regulating the activity of cullin-RING ligases." in: Nature cell biology, Vol. 12, Issue 4, pp. 351-61, 2010 (PubMed).

Caslini, Connelly, Serna, Broccoli, Hess: "MLL associates with telomeres and regulates telomeric repeat-containing RNA transcription." in: Molecular and cellular biology, Vol. 29, Issue 16, pp. 4519-26, 2009 (PubMed).

Shao, Fujii, Colmegna, Oishi, Goronzy, Weyand: "Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis." in: The Journal of experimental medicine, Vol. 206, Issue 6, pp. 1435-49, 2009 (PubMed).

Yalcin, Zhang, Luciano, Mungamuri, Marinkovic, Vercherat, Sarkar, Grisotto, Taneja, Ghaffari: "Foxo3 is essential for the regulation of ataxia telangiectasia mutated and oxidative stress-mediated homeostasis of hematopoietic stem cells." in: The Journal of biological chemistry, Vol. 283, Issue 37, pp. 25692-705, 2008 (PubMed).

Spardy, Duensing, Hoskins, Wells, Duensing: "HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies." in: Cancer research, Vol. 68, Issue 23, pp. 9954-63, 2008 (PubMed).

Luo, Rosenke, Czornak, Fortunato: "Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection." in: Journal of virology, Vol. 81, Issue 4, pp. 1934-50, 2007 (PubMed).

Bakkenist, Drissi, Wu, Kastan, Dome: "Disappearance of the telomere dysfunction-induced stress response in fully senescent cells." in: Cancer research, Vol. 64, Issue 11, pp. 3748-52, 2004 (PubMed).

Bakkenist, Kastan: "DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation." in: Nature, Vol. 421, Issue 6922, pp. 499-506, 2003 (PubMed).