DNA (Cytosine-5)-Methyltransferase 3A (DNMT3A) Antikörper

Antigen: DNA (Cytosine-5)-Methyltransferase 3A (DNMT3A)

Synonyme: DNMT3A2, M.HsaIIIA, W91664, AU015806, DNMT3A, MGC109195

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN151733

Produktbeschreibung

Weitere Bezeichnung: DNMT3A

Gen-ID: 1788

Swiss-Prot: Q9Y6K1

Immunogen: This antibody was raised against amino acids 10-118 ofthe human DNMT3a protein.

Format: Serum

Beschreibung: Inactivation of tumor suppressor genes is a key component of the development of allcommon forms of human cancers. Aberrant methylation may contribute to many of thechanges, both physiological and pathological, associated with aging, including tumordevelopment. Hypermethylation of CpG island-containing promoters is associated withthe inactivation of genes important to tumor angiogenesis and progression. DNAmethytransferase (DNMT3a) is necessary for the maintenance of the methylationprocess. Alternate Names: anti-DNMT3A2 antibody, anti-M.HsaIIIA antibody.

Spezifität: This antibody is specific for the DNMT3a protein. Species Reactivity: ABIN151733 recognizes human. Other species have notbeen tested. Localization: Nuclear.

Anwendungen

Applikationshinweise: Suggested working dilutions: immunoprecipitation 1:1,000,Western Blot 1:1,000. Positive Controls: HCT116 cell lysates

Buffer: 0.1 ml of rabbit antisera. This sera does not contain sodium azide or any otherpreservative.

Lagerung: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis using ABIN151733 to detect overexpressed DNMT3A level in HCT-116 cells. Lane 1: 40 ug of nuclear extract from untreated HCT-116 cells. Lane 2: 40 ug of Nuclear extract from HCT-116 cells overexpressing DNMT3A.

Publikationen

Publikationen: Beaulieu, Morin, Chute et al.: "An essential role for DNA methyltransferase DNMT3B in cancer cell survival." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28176-81, 2002 (PubMed).

Weisenberger, Velicescu, Preciado-Lopez et al.: "Identification and characterization of alternatively spliced variants of DNA methyltransferase 3a in mammalian cells." in: Gene, Vol. 298, Issue 1, pp. 91-9, 2002 (PubMed).

Robert, Morin, Beaulieu et al.: "DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells." in: Nature genetics, Vol. 33, Issue 1, pp. 61-5, 2003 (PubMed).

Reynolds, Sigaroudinia, Zardo et al.: "Tumor suppressor p16INK4A regulates polycomb-mediated DNA hypermethylation in human mammary epithelial cells." in: The Journal of biological chemistry, Vol. 281, Issue 34, pp. 24790-802, 2006 (PubMed).

Shikauchi, Saiura, Kubo et al.: "SALL3 interacts with DNMT3A and shows the ability to inhibit CpG island methylation in hepatocellular carcinoma." in: Molecular and cellular biology, Vol. 29, Issue 7, pp. 1944-58, 2009 (PubMed).