Claspin (CLSPN) Antikörper

Antigen: Claspin (CLSPN)

Synonyme: CLASPIN, MGC131612, MGC131613, MGC131615, CLSPN, xClaspin, C85083, B130025E01, E130314M08Rik, LOC398192

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN151714

Produktbeschreibung

Weitere Bezeichnung: Claspin (exons 11, 12, 13)

Gen-ID: 63967

Swiss-Prot: AAG245151

Immunogen: The epitope recognized by this antibody maps to a region between residues 450 and500 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967).

Beschreibung: Claspin is a cell cycle regulated protein. It plays a critical role in replication checkpointcontrol and interacts with the checkpoint proteins ATR and Rad9. It also associates withChk1, but only following replication stress or other types of DNA damage. Alternate Names: anti-Clspn antibody.

Spezifität: Species Reactivity: Human. Localization: Nucleus.

Anwendungen

Applikationshinweise: Suggested working dilutions: immunoprecipitation - 1 to 5 ug/ml of lysate,Western Blot - 1:5,000-1:20,000

Konzentration: 1 mg/ml

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8. Preservative: 0.09% Sodium Azide.

Lagerung: Store at 4C. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Detection of human Claspin by Western blot and Immunoprecipitation. Samples: HeLa Whole cell lysates (20 mcg/lane for WB, 1 mg for IP) Antibody: ABIN151714 used at the dilution shown for WB (A) and at 1 mcg/ml of lysate for IP and 0.2 mcg/ml for WB (B). Detection: Chemiluminescence with exposure time of 25 sec.

Publikationen

Publikationen: Li, Elder, Qin et al.: "Phosphatase type 2A-dependent and -independent pathways for ATR phosphorylation of Chk1." in: The Journal of biological chemistry, Vol. 282, Issue 10, pp. 7287-98, 2007 (PubMed).

Castriconi, Daga, Dondero et al.: "NK cells recognize and kill human glioblastoma cells with stem cell-like properties." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 6, pp. 3530-9, 2009 (PubMed).