Nucleoporin 214kDa (NUP214) Antikörper

Antigen: Nucleoporin 214kDa (NUP214)

Synonyme: CAN, CAIN, N214, D9S46E, MGC104525, RGD1304977, NUP214

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus

Applikation: Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN151667

Produktbeschreibung

Weitere Bezeichnung: NUP214

Gen-ID: 8021, 227720

Swiss-Prot: NP_0050763

Immunogen: The epitope recognized by this antibody maps to a region between residue 2050 andthe C-terminus (residue 2090) of human Nuceloporin 214 using the numbering given inentry NP_005076.3 (GeneID 8021).

Beschreibung: The nuclear pore complex is a massive structure that extends across the nuclearenvelope, forming a gateway that regulates the flow of macromolecules between thenucleus and the cytoplasm. Nucleoporins are the main components of the nuclear porecomplex in eukaryotic cells. This gene is a member of the FG-repeat-containingnucleoporins. The protein encoded by this gene is localized to the cytoplasmic face ofthe nuclear pore complex where it is required for proper cell cycle progression andnucleocytoplasmic transport. The 3', portion of this gene forms a fusion gene withthe DEK gene on chromosome 6 in a t(6,9) translocation associated with acute myeloidleukemia and myelodysplastic syndrome. Alternate Names: anti-N214 antibody, anti-N-214 antibody, anti-NUP214 antibody, anti-NUP-214 antibody,anti-Nucleoporin 214 antibody, anti-Nuclear Pore Complex Protien 214 antibody,anti-CAN antibody, anti-CAIN antibody.

Spezifität: Species Reactivity: Human and Mouse.

Anwendungen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.Prepare working dilution immediately before use.Suggested starting dilutions are as follows:*Western Blot: 1:2,000 to 1:10,000, Immunoprecipitation: 2 to 5 mcg/mg lysateImmunohistochemistry: not testedImmunocytochemistry: not tested*Investigator should determine optimal working conditions and dilutions.

Konzentration: 0.2 mg/ml

Buffer: Tris-buffered Saline containing 0.1% BSA. Preservative: 0.09% Sodium Azide.

Lagerung: Store at 4C. Do not freeze.

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Detection of Human and Mouse NUP214 by WB (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB, 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg), and mouse NIH3T3 (M, 50 mcg) cells. Antibodies: Affinity purified rabbit anti-NUP214 antibody ABIN151667 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B). NUP214 was also immunoprecipitated using rabbit anti-NUP214 antibody ABIN151667 at 3 mcg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Publikationen

Publikationen: Quentmeier, Schneider, Roehrs et al.: "SET-NUP214 fusion in acute myeloid leukemia- and T-cell acute lymphoblastic leukemia-derived cell lines." in: Journal of hematology & oncology, Vol. 2, pp. 3, 2009 (PubMed).