Structural Maintenance of Chromosomes 1A (SMC1A) (pSer966) Antikörper

Antigen: Structural Maintenance of Chromosomes 1A (SMC1A)

Synonyme: SMC1, SMCB, CDLS2, SB1.8, SMC1L1, DXS423E, KIAA0178, MGC138332, SMC1alpha, DKFZp686L19178, SMC-1A, smc1a, smc1l1, zgc:76902, im:7158706, SMC1A, SMC1L2, bK268H5, FLJ43748, SMC1BETA, bK268H5.5, NV15570

Epitope: pSer966

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN151528

Produktbeschreibung

Weitere Bezeichnung: SMC1A / SMC1 pSer966

Gen-ID: 8243

Swiss-Prot: NP_0062972

Immunogen: Immunogen for this antibody was a phosphorylated synthetic peptide, whichrepresented a portion of human Structural Maintenance of Chromosomes 1 (GeneID8243) around serine 966 according to the numbering given in entry NP_006297.2.

Beschreibung: Structural maintenance of chromosomes (SMC) proteins are part of the cohesin andcondensin multisubunit complexes that play a critical role in chromosome organization,segregation, gene regulation, and DNA repair. The core cohesin complex includes aheterodimer of SMC1 and SMC3 that functions by forming a ring-like structure that holdssister chromatids together. Cohesin is important to sister chromatid separation andsegregation during mitosis and meiosis and also plays a role in double-strand breakrepair by homologous recombination. Alternate Names: anti-SMC1L1-structural maintenance of chromosomes-1 like 1 antibody, anti-SMC-1antibody, anti-SMC 1 antibody.

Spezifität: Reacts with SMC1 phosphoSerine 966Phosphorylated:Ser966. Species Reactivity: Cross reacts with Human. Localization: Nuclear.

Anwendungen

Applikationshinweise: immunoprecipitation 2-6 ug/mg lysate,Western Blot 1:500-1:5000

Konzentration: 1 mg/ml

Buffer: Tris-buffered Saline containing 0.1% BSA. Preservative: 0.09% Sodium Azide.

Lagerung: Store at 4C. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis if human Phospho SMC1 (Ser966), using ABIN151528. Sample: Whole cell extracts from HeLa cells (30 ug/lane) that were irradiated with 10 Gy of ionizing radiation.

Publikationen

Publikationen: Leung-Pineda, Ryan, Piwnica-Worms: "Phosphorylation of Chk1 by ATR is antagonized by a Chk1-regulated protein phosphatase 2A circuit." in: Molecular and cellular biology, Vol. 26, Issue 20, pp. 7529-38, 2006 (PubMed).

Nahas, Butch, Du et al.: "Rapid flow cytometry-based structural maintenance of chromosomes 1 (SMC1) phosphorylation assay for identification of ataxia-telangiectasia homozygotes and heterozygotes." in: Clinical chemistry, Vol. 55, Issue 3, pp. 463-72, 2009 (PubMed).