Jagged 1 (JAG1) Antikörper

Antigen: Jagged 1 (JAG1)

Synonyme: AGS, AHD, AWS, HJ1, CD339, JAGL1, MGC104644, Htu, Ozz, Ser-1, slalom, Serrate-1, Headturner, JAG1

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human, Ratte (Rattus)

Applikation: Enzyme Immunoassay (EIA), Durchflusszytometrie (FACS), Neutralisierung (Neut), Western Blot (WB)

Produktnummer: ABIN150856

Produktbeschreibung

Weitere Bezeichnung: CD339 / JAG1

Immunogen: Recombinant rat jagged-1 extracellular domain expressed in mouse NSO cells.

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Jagged 1 is a notch ligand. The Notch signaling system regulates proliferation, differentiation and apoptosis in many tissues, including hematopoietic tissue. Notch is a family of transmembrane receptors activated by ligands such as jagged 1, jagged 2 and delta 1 that are expressed on adjacent cells. Hematopoietic stem cells and early progenitors express notch 1 and notch 2, whereas primary stromal cultures have been shown to express jagged 1, indicating that jagged 1 may play a role in nurturing progenitor cells within the hematopoietic microenvironment. Cells engineered to express high levels of jagged 1 caused a four-fold increase in formation of hematopoietic progenitor cell colonies. However, without the presence of other cytokines that stimulate proliferation and maturation, such as IL3, IL6, SCF and GCSF, jagged 1 actually decreased myeloid colony formation by CD34+ cells, suggesting that jagged 1 modulates cytokine signals and helps preserve CD34+ cells in an immature state.

Anwendungen

Applikationshinweise: ELISA: Use at a concentration of 0.5-1 ug/ml. The detection limit for recombinant rat Jagged-1 is approximately 0.16 ng/well. FACS: Use at a concentration of 20 ug/ml. (10 ul/10^5-10^6 cells) this concentration is recommended to label human Jagged 1 transfected FBM stromal cells. Neut: Use at a concentration of 2-10 ug/ml. The antibody will block 50% of the binding of 200 ng/ml of recombinant rat Jagged-1/Fc to immobilized recombinant rat Notch-1/Fc or recombinant rat Notch-2/Fc (100 ul of a 5 ug/ml solution coated in each well) in an ELISA assay. WB: Use at a concentration of 0.1-0.2 ug/ml, this concentration is determined using rat jagged-1 at 5 ng/lane under non-reducing conditions. Predicted molecular weight: 146 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Konzentration: 0.1 mg/ml

Reinheit: Immunogen affinity purified

Buffer: Preservative: NoneConstituents: PBS

Beschränkungen: Nur für Forschungszwecke einsetzbar