Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antikörper

Antigen: Actin, alpha 2, Smooth Muscle, Aorta (ACTA2)

Synonyme: AAT6, ACTSA, Actvs, a-SMA, SMalphaA, 0610041G09Rik, wu:fb63d03, MGC78870, ACTA2, actc1, actsa, MGC89192, MGC127800, ACT-4, DKFZp469E0314, Acta2, asma, acta, cftd, mpfd, nem1, nem2, nem3, acta2, cftd1, cftdm, MGC52636, MGC79039

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Huhn, Human, Maus, Ratte (Rattus)

Applikation: Enzyme Immunoassay (EIA), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN150426

Produktbeschreibung

Weitere Bezeichnung: Aortic smooth muscle Actin / ACTA2

Immunogen: Synthetic peptide (Human) corresponding to N-terminus of smooth muscle alpha actin.

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells, whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation , and altered by pathological conditions including oncogenic transformation and atherosclerosis.

Spezifität: This antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells. The other actins, such as bet

Synonyme: AAT6, ACTSA, Actvs, a-SMA, SMalphaA, 0610041G09Rik, wu:fb63d03, MGC78870, ACTA2, actc1, actsa, MGC89192, MGC127800, ACT-4, DKFZp469E0314, Acta2, asma, acta, cftd, mpfd, nem1, nem2, nem3, acta2, cftd1, cftdm, MGC52636, MGC79039

Anwendungen

Applikationshinweise: ELISA: Use at a concentration of 0.1-1.0 ug/ml. IHC: Use at a dilution of 1/100-1/400. WB: Use at a concentration of 0.5-2.0 ug/ml. Predicted molecular weight: 42 kDa. The formalin-fixed tissues were boiled in 10 mM citrate buffer, pH 6.0 for 10 min, followed by cooling at RT for 20 min. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Konzentration: 0.5 mg/ml

Reinheit: Immunogen affinity purified

Buffer: Preservative: 0.02% Sodium AzideConstituents: PBS. pH 7.4

Lagerung: Keep as concentrated solution, aliquot and store at 4C. Do not freeze.

Forschungsgebiet: Extrazelluläre Matrix, Zell-/Gewebemarker

Beschränkungen: Nur für Forschungszwecke einsetzbar