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Antikörper
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anti-Interleukin 17A (IL17A) Antikörper
Interleukin 17A (IL17A) Antikörper
| Antigen |
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| Synonyme |
IL17, CTLA8, IL-17, IL-17A, Il17, Ctla8, Ctla-8, IL17F, IL17A, ChIL-17 |
| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Applikation
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Durchflusszytometrie (FACS) (57), Western Blot (WB) (30), ELISA (23), Enzyme Immunoassay (EIA) (22), Neutralisierung (Neut) (22), Intrazelluläre Durchflusszytometrie (ICFC) (18), ELISA (Detektionsantikörper) (12), ELISPOT (11), ELISA (Fangantikörper) (8), Immunhistochemie (Paraffinschnitte) (IHC (p)) (7), Funktionale Studien (Func) (5), ELISPOT (Fangantikörper) (2), ELISPOT (Detektionsantikörper) (2), Immunfluoreszenz (IF) (2), Immunhistochemie (IHC) (2), Dot Blot (Dot) (1), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (1)
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| Produktnummer |
ABIN149188 |
| Menge |
0.1 mg (0.5 mg/ml) (Varianten) |
| Preis |
420,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Weitere Bezeichnung
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Interleukin-17A (IL17A)
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Immunogen
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Highly pure (>98%) recombinant hIL-17 (human Interleukin-17)
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Beschreibung
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Interleukin-17 was identified from a CD4+ T cell DNA library. IL-17 can be induced from primary peripheral blood CD4+ T cells upon stimulation. The cytokine exhibits a high degree of amino acid identity with HVS13, an open reading frame from a T lymphotropic Herpes virus saimiri and with murine CTLA8. Supernatant from cells transfected with IL-17 induced IL-6 and IL-8 production and enhanced the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. IL-17 is a disulfide-linked homodimer of two 15.5 kDa subunits, each containing 136 amino acid residues.
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Applikationshinweise
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Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17 (6.0 ng/ml), a concentration of 0.1-0.18 ug/ml of this antibody is required. ELISA:To detect hIL-17 by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant hIL-17. Western Blot:To detect hIL-17 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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Konzentration
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0.5 mg/ml
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Reinheit
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Immunogen affinity purified
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Buffer
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PBS, pH 7.4, no preservative, sterile filtered
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Lagerung
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Store at 4 C.
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Forschungsgebiet
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Zytokine, Rezeptoren, Inflammation, Immunologie, Virologie, Krebs
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Interleukin 17A (IL17A)", Typ "Antikörper" finden
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Wirte
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Maus (88), Ratte (27), Kaninchen (16), Ziege (5), Huhn (1)
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Reaktivitäten
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Human (104), Maus (34), Ratte (Rattus) (3)
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Applikationen
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Durchflusszytometrie (FACS) (57), Western Blot (WB) (30), ELISA (23), Enzyme Immunoassay (EIA) (22), Neutralisierung (Neut) (22), Intrazelluläre Durchflusszytometrie (ICFC) (18), ELISA (Detektionsantikörper) (12), ELISPOT (11), ELISA (Fangantikörper) (8), Immunhistochemie (Paraffinschnitte) (IHC (p)) (7), Funktionale Studien (Func) (5), ELISPOT (Fangantikörper) (2), ELISPOT (Detektionsantikörper) (2), Immunfluoreszenz (IF) (2), Immunhistochemie (IHC) (2), Dot Blot (Dot) (1), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (1)
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Konjugate
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Biotin (24), Alexa Fluor 647 (9), HRP (8), FITC (7), PE (7), PerCP,Cy5.5 (6), Alexa Fluor 488 (5), Pacific Blue (4), APC (1)
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Epitope
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N-Term (2), AA 20-155 (1), Center (1)
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Alternativen 
