Serotonin (5-HT) Antikörper

Antigen: Serotonin (5-HT)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus)

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN148457

Produktbeschreibung

Immunogen: Serotonin coupled to bovine serum albumin with paraformaldehyde.

Format: Serum

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Carcinoid tumours are the most commonly occurring endocrine tumours. Although generally found in the gut wall, they also occur in the pancreas, rectum, ovary and lung. Carcinoid tumours typically contain numerous membrane bound neurosecretory granules, which are composed of a variety of hormones and biogenic amines. One of the best characterised of these substances is serotonin. Serotonin is synthesized from its precursor, 5 hydroxytrytophan, by the enzyme aromatic acid decarboxylase. Serotonin is subsequently metabolised by monoamine oxidase to 5 hydroxyindoleacetic acid, which is excreted in the urine. In addition to serotonin, carcinoid tumours have been found to secrete corticotropin, histamine, dopamine, substance P, neurotensin, prostaglandins, and kallikrein. Among endocrine tumours of the gastrointestinal tract, carcinoid tumours arising from the midgut secrete serotonin, while carcinoid tumours arising from the hindgut rarely secrete serotonin.

Anwendungen

Applikationshinweise: IHC-P: Use at a dilution of 1/100-1/200. IHC-Fr: Use at a dilution of 1/100-1/200. Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode?s solution, 2) paraformaldehye-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18 24 hours at 4°,C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. Note: Sodium azide (NaN3) interferes with peroxidase reactions and should not be used with peroxidase methodologies. If sodium azide is present in any steps of the staining procedure, the tissue should thoroughly be rinsed with sodium azide free buffer before performing the peroxidase reaction. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Reinheit: Whole antiserum

Buffer: Preservative: 15mM Sodium AzideConstituents: 1% BSA, Whole Serum

Lagerung: Store at 4 C. Aliquot and store at -20 C long-term.

Forschungsgebiet: Hormone

Beschränkungen: Nur für Forschungszwecke einsetzbar