Calnexin (CANX) Antikörper

Antigen: Calnexin (CANX)

Synonyme: CNX, P90, IP90, FLJ26570, Cnx, AI988026, D11Ertd153e, 1110069N15Rik, DmelCG11958, CG11958, canx, MGC52927, CANX, PP90, zgc:63524, wu:fe06b12, MGC166317, MGC69400, fj49d10, MGC153946, wu:fj24b04, wu:fj49d10, zgc:153946, DKFZp459P042, LOC100283947

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Rind (Kuh), Hund, Huhn, Fruchtfliege (Drosophila melanogaster), Meerschweinchen, Hamster, Human, Affe, Maus, Schwein, Kaninchen, Ratte (Rattus), Schaf, Xenopus laevis

Applikation: Durchflusszytometrie (FACS), Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN148412

Produktbeschreibung

Weitere Bezeichnung: Calnexin

Immunogen: Synthetic peptide: EEDEILNRSPRNRKPRRE conjugated to KLH, corresponding to amino acids 575-593 of Human Calnexin.

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains

Synonyme: CNX, P90, IP90, FLJ26570, Cnx, AI988026, D11Ertd153e, 1110069N15Rik, DmelCG11958, CG11958, canx, MGC52927, CANX, PP90, zgc:63524, wu:fe06b12, MGC166317, MGC69400, fj49d10, MGC153946, wu:fj24b04, wu:fj49d10, zgc:153946, DKFZp459P042, LOC100283947

Anwendungen

Applikationshinweise: FACS: Use at a dilution of 1/100. ICC: Use at a dilution of 1/200. IP: Use at a dilution of 1/100. WB: Use at a dilution of 1/2000 (this dilution was sufficient for detection of Calnexin in 20ug of HeLa heat shocked cell lysate). Detects a band of approximately 90 kDa. (predicted molecular weight: 71 kDa). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Reinheit: Whole antiserum

Buffer: Preservative: NoneConstituents: Whole Serum

Lagerung: Store at 4 C. Aliquot and store at -20 C long-term.

Beschränkungen: Nur für Forschungszwecke einsetzbar