6xHistidine Epitope Tag Antikörper

Antigen: 6xHistidine Epitope Tag

Klonalität: Polyklonal

Wirt: Huhn

Applikation: Enzyme Immunoassay (EIA), Western Blot (WB)

Produktnummer: ABIN148405

Produktbeschreibung

Weitere Bezeichnung: 6xHistidine Epitope Tag (HHHHHH)

Immunogen: Synthetic peptide: HHHHHH conjugated to KLH.

Beschreibung: This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.

Spezifität: Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:8000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using Goat Anti Chicken IgY (IgG) and TMB as the substrate. Appropriate specificity control

Anwendungen

Applikationshinweise: This antibody was an effective at 1:1000 (1 ug/ml) dilution using an affinity purified goat anti chicken IgY (IgG) HRP. The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding.

Konzentration: 1 mg/ml

Reinheit: IgY fraction

Buffer: PBS, pH 7.2, sodium azide, 0.1%

Lagerung: Store at 4 C.

Beschränkungen: Nur für Forschungszwecke einsetzbar