LIM Domain Binding 1 (LDB1) Antikörper

Antigen: LIM Domain Binding 1 (LDB1)

Synonyme: NLI, CLIM2, chi, nli, clim2, xldb1, MGC53474, LDB1

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Huhn, Human, Maus, Xenopus laevis

Applikation: Chromatin-Immunopräzipitation (ChiP)

Produktnummer: ABIN147683

Produktbeschreibung

Weitere Bezeichnung: LDB1

Immunogen: Synthetic peptide: GSQESKSENPTSQA, corresponding to amino acids 361-373 of Mouse LDB 1.

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB 1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development.

Spezifität: ChIP testing conditions:The Ldb1 antibody has been used in a ChIP assay in murine erythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1 gene, studies from the Orkin and Rabbitts? groups have suggested that Ldb1 binds to th

Anwendungen

Applikationshinweise: ChIP: Use 20ug for 10^8 cells. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Konzentration: 1 mg/ml

Reinheit: Immunogen affinity purified

Buffer: Preservative: 0.01% Sodium AzideConstituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2

Lagerung: Store at 4 C. Aliquot and store at -20 C long-term.

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, Transkriptionsfaktoren, Neurogenese

Beschränkungen: Nur für Forschungszwecke einsetzbar