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Antikörper
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anti-phosphodiesterase 4D, CAMP-Specific (PDE4D) Antikörper
phosphodiesterase 4D, CAMP-Specific (PDE4D) Antikörper
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| Synonyme |
DPDE3, STRK1, HSPDE4D, PDE4DN2, FLJ97311, DKFZp686M11213 |
| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Applikation
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 Alternativen Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB)
Immunfluoreszenz (IF) (22), Western Blot (WB) (22), Durchflusszytometrie (FACS) (19), Enzyme Immunoassay (EIA) (9), Immunpräzipitation (IP) (8), ELISA (5), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (4), Immunhistochemie (Paraffinschnitte) (IHC (p)) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (IHC) (1)
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| Produktnummer |
ABIN147651 |
| Menge |
0.1 mg (0.8 mg/ml) (Varianten) |
| Preis |
450,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Weitere Bezeichnung
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PDE4D
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Immunogen
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C-terminal cyclic peptide unique to all PDE4D subtypes variants.
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Isotyp
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IgG (Passende Sekundärantikörper)
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Beschreibung
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Cyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprise of 5 variants (PDE4D1, D2, D3, D4 and D5). One or more PDE4D subtype-variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype-variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short-term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein-protein interactions with cytoskeletal scaffolding proteins.
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Spezifität
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ABIN147651 labels all PDE4D proteins including PDE4D1, PDE4D2, PDE4D3, PDE4D4, PDE4D5 variants. Does not cross react with PDE4A, PDE4B or PDE4C.
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Applikationshinweise
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ICC: Use at a dilution of 1/150. IP: Use at a dilution of 1/150. WB: Use at a dilution of 1/500. Detects a band of approximately 91 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Konzentration
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0.8 mg/ml
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Reinheit
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Immunogen affinity purified
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Buffer
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Preservative: 0.02% Thimerosal / Merthiolate
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Lagerung
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Store at 4 C. Aliquot and store at -20 C long-term.
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Forschungsgebiet
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Signaltransduktion, Enzyme, Metabolismus
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "phosphodiesterase 4D, CAMP-Specific (PDE4D)", Typ "Antikörper" finden
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Wirte
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Kaninchen (32), Ziege (8), Maus (1)
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Reaktivitäten
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Human (35), Maus (27), Ratte (Rattus) (27), Hund (2), Pferd (2), Huhn (1), Rind (Kuh) (1), Schwein (1), Schaf (1)
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Applikationen
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Immunfluoreszenz (IF) (22), Western Blot (WB) (22), Durchflusszytometrie (FACS) (19), Enzyme Immunoassay (EIA) (9), Immunpräzipitation (IP) (8), ELISA (5), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (4), Immunhistochemie (Paraffinschnitte) (IHC (p)) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (IHC) (1)
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Konjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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C-Term (1), pSer190 (1)
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