Activin A Receptor, Type IIA (ACVR2A) Antikörper

Antigen: Activin A Receptor, Type IIA (ACVR2A)

Synonyme: ACVR2, ACTRII, Acvr2, ActrIIa, TactrII, rActR-II, ACVR2A, acvr2a, acvr2, actr2a, wu:fa02e10, actrii

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: Enzyme Immunoassay (EIA), Neutralisierung (Neut), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN147291

Produktbeschreibung

Weitere Bezeichnung: Activin receptor type 2A (ACVR2A)

Immunogen: Recombinant fragment (Human).

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Activin, a disulfide-linked homodimeric protein is secreted by Sertoli cells in the testis and granulosa cells in the ovary. In early studies, this peptide was thought to be an inhibin and not recognized as a unique compound. Activins and inhibins are members of the TGF-beta superfamily due to amino acid homology with respect to the conservation of 7 of the 9 cysteine residues common to all TGF-beta forms. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits have been cloned (mammalian betaA, betaB, betaC, betaE, and Xenopus betaD). The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A (betaA-betaA), activin B (betaB-betaB), activin AB (betaB-betaA), inhibin A (alpha-betaA), and inhibin B (alpha- betaB). Activins have a wide range of biological activities including mesoderm induction, neural cell differenti

Spezifität: The antibody exhibits less than 2 % cross-reactivity with recombinant human activin receptor I and recombinant human activin receptor IIB.

Anwendungen

Applikationshinweise: ELISA: Use at a concentration of 0.5-1.0 ug/ml. IHC: Use at a concentration of 15 ug/ml. Neut: Used at a concentration of 1-3 ug/ml, the antibody will block 50 % of the binding of 30 ng/ml recombinant human activin A to immobilized recombinant human activin receptor IIA/Fc chimera (100 ul of a 0.5 ug/ml solution coating each well) in a functional ELISA assay. WB: Use at a concentration of 0.1-0.2 ug/ml. Detects human activin receptor IIA at approximately 5 ng/lane under reducing and non-reducing conditions. Predicted molecular weight: 58 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Konzentration: 0.1 mg/ml

Reinheit: Immunogen affinity purified

Buffer: Preservative: NoneConstituents: PBS

Forschungsgebiet: Rezeptoren

Beschränkungen: Nur für Forschungszwecke einsetzbar