phosphodiesterase 4B, CAMP-Specific (PDE4B) Antikörper

Antigen: phosphodiesterase 4B, CAMP-Specific (PDE4B)

Synonyme: DPDE4, PDE4B5, PDEIVB, MGC126529, DKFZp686F2182, MGC83972, MGC147384

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN146981

Produktbeschreibung

Weitere Bezeichnung: PDE4B

Immunogen: Synthetic peptide (C terminal).

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. Four genes (PDE4A, B, C and D) make up the PDE4 family. cAMP-dependent phosphodiesterase type B (PDE4B) family consists of four known splice variants, PDE4B1, B2, B3 and a novel 66 kDa PDE4B4 protein. One or more subtype variants of PDE4B are ubiquitously present in all mammalian cells. In CNS, all four PDE4B subtype variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Some PDE4B variants are also substrate for PKA-dependent phosphorylation. Peripheral tissues exhibit differential expression of PDE4B1, B2 and B4 variants, the PDE4B4 (66kDa protein) is the major PDE4B variant expressed in lungs, testis and heart. In testis PDE4B enzymes are expressed in somatic cells (Sertoli and Leydig cells) while PDE4A is localized in germ cells.

Anwendungen

Applikationshinweise: ICC: Use at a dilution of 1/300. IF: Use at a dilution of 1/200. IP: Use at a dilution of 1/350. WB: Use at a dilution of 1/1500. Detects bands of 107 kDa (PDE4B1), 78 kDa (PDE4B2), 100 kDa (PDE4B3) and 66 kDa (PDE4B4). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Konzentration: 0.65 mg/ml

Reinheit: Immunogen affinity purified

Buffer: Preservative: NoneConstituents: 20% Glycerol, PBS

Lagerung: Store at 4 C. Aliquot and store at -20 C long-term.

Forschungsgebiet: Signaltransduktion, Enzyme, Metabolismus

Beschränkungen: Nur für Forschungszwecke einsetzbar