14-3-3 Binding Motif (pSer) Antikörper

Antigen: 14-3-3 Binding Motif

Epitope: pSer

Klonalität: Polyklonal

Wirt: Maus

Applikation: Enzyme Immunoassay (EIA), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN146343

Produktbeschreibung

Weitere Bezeichnung: 14-3-3 Binding Motif pSer

Immunogen: Synthetic phospho(Ser) 14-3-3 binding motif peptides (KLH coupled).

Isotyp: IgG1  (Passende Sekundärantikörper)

Beschreibung: This antibody provides a powerful tool for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery.

Spezifität: ABIN146343 binds peptides/ proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. By ELISA the antibody recognizes a wide range of peptides containing this phosphorylated 14-3-3 binding motif in a manner that is

Anwendungen

Applikationshinweise: ELISA: Use at a dilution of 1/1000. IP: Use at a dilution of 1/20 on denatured proteins. WB: Use at a dilution of 1/4000. Predicted molecular weight: 30 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Reinheit: Protein A purified

Buffer: Preservative: 0.02% sodium azide, Constituents: 50% Glycerol, BSA, 150mM Sodium Chloride, 10mM HEPES. pH 7.5

Beschränkungen: Nur für Forschungszwecke einsetzbar