FBL Antikörper

Antigen: FBL

Klonalität: Polyklonal

Wirt: Maus

Reaktivität: Caenorhabditis elegans (C. elegans), Fruchtfliege (Drosophila melanogaster), Human, Maus, Ratte (Rattus), Yeast (Pichia pastoris)

Applikation: Immunfluoreszenz (IF), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN145633

Produktbeschreibung

Immunogen: Yeast nuclear preparation (S. cerevisiae).Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae).

Format: Supernatant

Isotyp: IgG1  (Passende Sekundärantikörper)

Beschreibung: Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologues to the other sequences throughout all

Anwendungen

Applikationshinweise: ICC: Use at an assay dependant dilution (to identify nucleoli). IF: Use at a dilution of 1/1000-1/5000 (yeast cells). For IF of mammalian cells, use at a dilution of 1/500. WB: Use at a dilution of 1/2000 (cell lysates)-1/10000 (nuclear fractions)(ECL). Detects a band of approximately 34 kDa and shows a clear and strong punctate staining of yeast nuclei. For other (non-ECL) western detection methods, use at a dilution of 1/1000-1/5000. To detect mammalian fibrillarin on western blots by ECL, use at a dilution of 1/500. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Reinheit: Tissue culture supernatant

Buffer: Preservative: 10mM Sodium Azide, Constituents: Tissue Culture Supernatant

Lagerung: Store at 4 degrees C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-FBL antibody