CNPase (CNP) Antikörper

Antigen: CNPase (CNP)

Synonyme: CNP1, Cnp1, Cnp-1, CNPase, CNPF, CNPI, CNPII, CNP, sb:cb662, wu:fd44a05, wu:fi35d08, si:ch73-158e11.4, DKFZp469F1421

Klonalität: Polyklonal

Wirt: Maus

Reaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Kaninchen, Ratte (Rattus), Schaf

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Dot Blot (Dot), Enzyme Immunoassay (EIA), Immunfluoreszenz (IF), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN144291

Produktbeschreibung

Weitere Bezeichnung: CNPase

Immunogen: Full length native protein (purified) (Human).

Format: Ascites

Isotyp: IgG1  (Passende Sekundärantikörper)

Beschreibung: CNPase (2,3-Cyclic Nucleotide 3-Phosphodiesterase) exists in two forms: 46kDa (CNP 1) and 48kDa (CNP 2). It is expressed at high levels by oligodendrocytes in the central nervous system and by Schwann cells in the peripheral nervous system and is virtual absent in other celltypes. CNPase activity is decreased in demyelinating diseases such as multiple sclerosis. CNPase gene is localized to chromosome 17q21, very near the BRCA 1 familial breast cancer gene.

Spezifität: Reacts specifically with CNPase. In an immunoblotting assay, the antibody localizes both CNP1 (46kD) and CNP2 (48kD) bands.Immunohistochemical staining of paraffin, cryostat or vibratome sections of rat brain reveals selective staining of oligodendrocytes

Anwendungen

Applikationshinweise: Conjugation: Use at an assay dependant dilution. Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/500 for immunoblotting of fresh bovine whole brain extractNot tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Konzentration: ascites

Reinheit: Ascites

Buffer: Preservative: 0.1% Sodium AzideConstituents: Ascites fluid

Lagerung: "Store at 4 C or -20 C long term. Repeated freezing and thawing is not recommended. Storage in """"frost-free"""" freezers is not recommended. """"

Forschungsgebiet: Neurologie, Glia Marker

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Sprinkle, Agee, Tippins et al.: "Monoclonal antibody production to human and bovine 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNPase): high-specificity recognition in whole brain acetone powders and conservation of sequence between CNP1 and CNP2." in: Brain research, Vol. 426, Issue 2, pp. 349-57, 1988 (PubMed).

Reynolds, Carey, Herschkowitz: "Immunohistochemical localization of myelin basic protein and 2',3'-cyclic nucleotide 3'-phosphohydrolase in flattened membrane expansions produced by cultured oligodendrocytes." in: Neuroscience, Vol. 28, Issue 1, pp. 181-8, 1989 (PubMed).

Sprinkle: "2',3'-cyclic nucleotide 3'-phosphodiesterase, an oligodendrocyte-Schwann cell and myelin-associated enzyme of the nervous system." in: Critical reviews in neurobiology, Vol. 4, Issue 3, pp. 235-301, 1989 (PubMed).