Histone H1 Antikörper

Antigen: Histone H1

Synonyme: H1c, H1.2, H1f2, His1a, H1var1, MGC107646, 0610008C09Rik, LOC692942, HISTONE H1, HON4

Klonalität: Polyklonal

Wirt: Maus

Reaktivität: Human

Applikation: Chromatin-Immunopräzipitation (ChiP), Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN144214

Produktbeschreibung

Immunogen: Nuclei from myeloid leukemia biopsy cells.

Isotyp: IgG2a  (Passende Sekundärantikörper)

Beschreibung: Although histones are classified into a limited number of types of fractions with each particular fraction having a fundamentally distinct amino acid composition and sequence, numerous subfractions are observed due to the acetylation, methylation, and phosphorylation of various amino acid residues. Microheterogeneity or alteration of structure is dynamic such that the histones of a single cell type are found to vary during development. They are believed to play a role in gene activity and cellular metabolism. Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. With the exception of H1, the primary structures of the calf thymus histones have been determined. Comparisons with the structures for histones from other sources indic

Spezifität: Doublet detected due to hyper-phosphorylation of histone H1 subpopulation.

Anwendungen

Applikationshinweise: ChIP: Use 4-5ug for 10^6 cells. ICC: Use at a concentration of 5-20 mg/ml. WB: Use at a concentration of 1-4 ug/ml. Predicted molecular weight: 32-33 kDa. Detects histone H1 in lysates from an acid extract of HeLa cells. Lysate from acid extracted HeLa cells was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Histone H1 (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Konzentration: 1 mg/ml

Reinheit: Protein A purified

Buffer: PBS with 0.1% sodium azide, 40% glycerol

Lagerung: Antibody may be stored at 4 C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20 C. Aliquots are stable for 24 months at -20 C. For maximum recovery of product, centrifuge the original vial af

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, DNS/RNS, Transkriptionsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Monvall: "[Students' action adopted at convention]" in: Tidskrift för Sveriges sjuksköterskor, Vol. 42, Issue 22, pp. 23-4, 1976 (PubMed).

Hebbes, Thorne, Crane-Robinson: "A direct link between core histone acetylation and transcriptionally active chromatin." in: The EMBO journal, Vol. 7, Issue 5, pp. 1395-402, 1988 (PubMed).