Acetylated Lysine Antikörper

Antigen: Acetylated Lysine

Klonalität: Polyklonal

Wirt: Schaf

Reaktivität: Human, Yeast (Pichia pastoris)

Applikation: Enzyme Immunoassay (EIA), Western Blot (WB)

Produktnummer: ABIN141769

Produktbeschreibung

Immunogen: Tetra-acetylated N-terminal peptide corresponding to aa 1-18 of human histone H4

Beschreibung: Immunohistochemistry showed that the antiserum stains human and mouse chromosome preparations in a pattern that correlates with the acetylation as established by other means (unpublished data) and is equivalent to the rabbit polyclonal described in Turner & Fellows.

Spezifität: The antibody has also been shown to react against histones, PCAF and E2F in Western blotting. Its suitability as a pan-specific anti-acetylated lysine antibody is currently under evaluation.

Anwendungen

Applikationshinweise: Flow cytometry / FACS: assay dependent. Western Blotting: use at a concentration of 1-2ug/ml for 2hrs at RT. Immunohistochemistry: use at a concentration of 1-2ug/ml for 30 min at RT. Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 62 kDa, which corresponds to the predicted molecular weight of Cyclin B1.

Konzentration: 1

Reinheit: Whole antisera

Buffer: Phosphate buffered saline

Lagerung: Store at 4 C. Long term (>6months) aliquot and store at -20 C. Avoid repeated freeze thawing. :

Beschränkungen: Nur für Forschungszwecke einsetzbar