V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) (EQKLISEEDL) Antikörper

Antigen: V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC)

Synonyme: MRTL, c-Myc, bHLHe39, MYC, CMYC, MGC138120, c-myc, C-MYC, LOC100136746

Epitope: EQKLISEEDL

Klonalität: Polyklonal

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), Durchflusszytometrie (FACS), Funktionale Studien (Func), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunelektronenmikroskopie (EM)

Produktnummer: ABIN137648

Produktbeschreibung

Weitere Bezeichnung: C-Myc Epitope Tag (EQKLISEEDL)

Immunogen: Synthetic peptide: AYILSVQAEEQKLISEEDLL conjugated to KLH, corresponding to amino acids 408-439 of Human Myc tag.

Isotyp: IgG1  (Passende Sekundärantikörper)

Beschreibung: Excellent for immunoprecipitation and Western blotting of fusion proteins labeled with EQKLISEEDL tag. 9E10 recognises recombinant proteins incorporating a c-Myc epitope tag. 9E10 also detects human c-Myc protein and a peptide in random coil configuration not as a helix.

Spezifität: The antibody recognises the c-myc peptide in random coil configuration, not as a helix. 9E10 does not react with v-myc.

Anwendungen

Applikationshinweise: AF: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. FACS: Use at an assay dependent dilution. IHC-F: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. P: Use at an assay dependent dilution. WB: Use at a dilution of 1:500-1:1,000. Histology: use at 1:250-1:500 (30-60 minutes at RT). This antibody has been used for immunolocalisation with gold conjugated secondaries on plant tissue. This antibody is not suitable for immunoprecipitation of native c-myc protein. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Konzentration: 1.2 mg/ml

Reinheit: Protein A purified

Buffer: Phosphate-buffered saline

Lagerung: Keep as concentrated solution. Store at 4?C short term. For extended storage aliquot and store at -20?C or below. Avoid freeze-thaw cycles.

Forschungsgebiet: Transkriptionsfaktoren, Tags/Labels

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Royds, Sharrard, Wagner et al.: "Cellular localisation of c-myc product in human colorectal epithelial neoplasia." in: The Journal of pathology, Vol. 166, Issue 3, pp. 225-33, 1992 (PubMed).

Kari, Lussenhop, Goertz et al.: "Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes." in: Journal of virology, Vol. 60, Issue 2, pp. 345-52, 1986 (PubMed).

Spandidos, Pintzas, Kakkanas et al.: "Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue." in: Anticancer research, Vol. 7, Issue 6, pp. 1299-304, 1988 (PubMed).

Evan, Lewis, Ramsay et al.: "Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product." in: Molecular and cellular biology, Vol. 5, Issue 12, pp. 3610-6, 1986 (PubMed).

Baumberger, Ringli, Keller: "The chimeric leucine-rich repeat/extensin cell wall protein LRX1 is required for root hair morphogenesis in Arabidopsis thaliana." in: Genes & development, Vol. 15, Issue 9, pp. 1128-39, 2001 (PubMed).