Asialoganglioside GM1 Antikörper

Antigen: Asialoganglioside GM1

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus, Ratte (Rattus)

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Abreicherung (Depletion)

Zertifikate: ISO 9001:2008, ISO 13485:2003

Produktnummer: ABIN133999

Produktbeschreibung

Weitere Bezeichnung: Asialo GM1

Immunogen: Rabbits were immunized with asialo GM1 purified from bovine brain tissue, methylated bovine serum albumin and complete Freund’s adjuvant.

Format: Purified

Isotyp: Ig Fraction  (Passende Sekundärantikörper)

Beschreibung: The Anti-Asialo GM1 polyclonal antibody reacts with mouse and rat Natural Killer (NK) cells. It also exhibits slight reactivity with mouse monocytes (liver cells which contain no NK cells; bone marrow; fetal liver cells; spleen cells of nude mice), macrophages, and fetal thymocytes (12 days old; ratio of existence decreased gradually until there were none in newborn mice). The anti-Asialo GM1 antiserum has been shown to eliminate NK activity in cells of various strains of mice and rats.

Anwendungen

Applikationshinweise: Applications include: flow cytometry, immunohistochemistry (frozen sections and formalin-fixed paraffin embedded sections), immunoprecipitation and NK cell depletion. Antibody Titre: Approximately 1:1000 by immunoflocculation test.

Konzentration: Total Protein Concentration: 37 mg/ml. Albumin Concentration: 9 mg/ml.

Reinheit: Since this antibody is not a purified product, the protein concentration is not equal to the concentration of Asialo-GM1-specific antibody, even after correcting for the albumin content. Both the total protein and albumin concentrations will vary from lot to lot.

Reinigung: The gammaglobulin fraction of serum was obtained by 50% ammonium sulfate precipitation followed by dialysis with phosphate buffered saline (pH 7.2).

Lagerung: RECONSTITUTION: Distilled water is recommended (1 ml). Since the material is lyophilized with salts, use of other solvents such as PBS or MEM may increase the salt concentration. Store at 4°C before reconstitution: stable for 2 years when refrigerated. After reconstitution: stable for 2-3 months when refrigerated, 2-3 days at room temperature. DO NOT FREEZE.

Beschränkungen: Nur für Forschungszwecke einsetzbar