MAC-2 Antikörper

Antigen: MAC-2

Klonalität: Monoklonal

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS)

Zertifikate: ISO 9001:2008, ISO 13485:2003

Produktnummer: ABIN133960

Produktbeschreibung

Immunogen: Plasma membrane glycoproteins from C57BL/6 mouse thioglycollate-elicited peritoneal exudate. . Partner: NS-1 . Donor: (Lewis x BN) F rat spleen Fusion

Format: Purified

Isotyp: IgG2a  (Passende Sekundärantikörper)

Beschreibung: Anti-Mac-2 monoclonal antibody specifically binds the mouse Mac-2 antigen (also cross reacts with human). The antibody recognizes a 32,000 dalton surface antigen found on a subpopulation of mouse macrophages. It reacts with peritoneal exudate macrophages where the exudate is provoked by thioglycollate, protease peptone (20%), macrophages of lymphoid and non-lymphoid tissues, interdigitating dendritic cells and Langerhans cells. Mac-2 is also expressed in the cytoplasm on non-elicited resident macrophages 5% are strongly reactive, the remaining 95% show much weaker staining. The antibody does not react with peritoneal exudate macrophages where the exudate is provoked by Listeria monocytogenes, lipopolysaccharide or concanavalin A. It also does not react with peritoneal macrophages, splenic macrophages, granulocytes, thymocytes, peripheral lymph node cells and with 99% of bone marrow cells. ABIN133960can be used for indirect immunofluorescence staining, including flow cytometric analysis of live cells. The addition of propidium iodide is optional its use eliminates staining artifacts caused by dead cells (1). This antibody is also suitable for frozen sections (4,5) ELISA (6) and paraffin sections (7).

Spezifität: Mouse MAC-2

Anwendungen

Lagerung: For all formats, store at 4°C. DO NOT FREEZE PE conjugates. For long term storage (Purified, Biotin, FITC, No Azide), aliquot and freeze unused portion at -20 °C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar