Interleukin 1, beta (IL1B) Antikörper (FITC)

Antigen: Interleukin 1, beta (IL1B)

Synonyme: IL-1, IL1F2, IL1-BETA, IL1B, IL-1BETA, MGC111873, zgc:111873, IL-1B

Klonalität: Monoklonal

Wirt: Maus

Reaktivität: Human

Konjugat: FITC

Applikation: Immunfluoreszenz (IF), Durchflusszytometrie (FACS)

Produktnummer: ABIN132135

Produktbeschreibung

Weitere Bezeichnung: IL-1 beta

Format: Purified

Isotyp: IgG1

Spezifität: IL-1? is strongly expressed upon activation of monocytes and macrophages. IL-1? affects a wide range of cells and organs. Ist spectrum of action is similar to that of TNF. The IL-1? mAb (clone FIB3) reacts with intracellular human IL-1? expressed by activated monocytes/macrophages or dendritic cells.

Anwendungen

Applikationshinweise: The FIB3 antibody permits the analysis of activated human monocytes, macrophages as well as myeloid dendritic cells using flow cytometry. To allow intracellular accumulation of IL-1? (prevent secretion), cells of interest can be pretreated with e.g. Brefeldin A (2 ?g/ml) for 18 hours before analyses. Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained which cannot be attributed to differences in laboratory procedures, please contact us. Samples: Biological fluids (blood, bone marrow, and others) must be collected under sterile conditions. Anticoagulation with EDTA or heparin is recommended. The samples should be stored at room temperature until used. For optimal results, samples should be processed and analyzed within 24 hours. Samples with high numbers of non-viable cells might cause false results, such cases require determination of cell viability with e.g. propidium iodide. All biological samples have to be handled with caution. Always consider them as potentially infective. Use appropriate precautions such as gloves, lab-coat, etc. Sensitivity: The sensitivity of FIB3 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAbconcentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ?l of leukocytes containing 107cells/ml are stained with 20 ?l mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.

Reinigung: Chromatography

Buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3

Lagerung: For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

anti-Interleukin 1, beta (IL1B) antibody (FITC)

Publikationen

Publikationen: Chikanza, Corrigal, Kingsley et al.: "Enumeration of interleukin-1 alpha and beta producing cells by flow cytometry." in: Journal of immunological methods, Vol. 154, Issue 2, pp. 173-8, 1992 (PubMed).

Rubartelli, Cozzolino, Talio et al.: "A novel secretory pathway for interleukin-1 beta, a protein lacking a signal sequence." in: The EMBO journal, Vol. 9, Issue 5, pp. 1503-10, 1990 (PubMed).

Conlon, Grabstein, Alpert et al.: "Localization of human mononuclear cell interleukin 1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 1, pp. 98-102, 1987 (PubMed).

Auron, Webb, Rosenwasser et al.: "Nucleotide sequence of human monocyte interleukin 1 precursor cDNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 24, pp. 7907-11, 1985 (PubMed).

Waclavicek, Majdic, Stulnig et al.: "CD99 engagement on human peripheral blood T cells results in TCR/CD3-dependent cellular activation and allows for Th1-restricted cytokine production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 9, pp. 4671-8, 1998 (PubMed).

Bueno, Almeida, Alguero et al.: "Flow cytometric analysis of cytokine production by normal human peripheral blood dendritic cells and monocytes: comparative analysis of different stimuli, secretion-blocking agents and incubation periods." in: Cytometry, Vol. 46, Issue 1, pp. 33-40, 2001 (PubMed).

Schuerwegh, Stevens, Bridts et al.: "Evaluation of monensin and brefeldin A for flow cytometric determination of interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha in monocytes." in: Cytometry, Vol. 46, Issue 3, pp. 172-6, 2001 (PubMed).