CD68 (CD68) Antikörper (FITC)

Antigen: CD68 (CD68)

Synonyme: GP110, SCARD1, DKFZp686M18236

Klonalität: Monoklonal

Wirt: Maus

Reaktivität: Human

Konjugat: FITC

Applikation: Immunfluoreszenz (IF), Durchflusszytometrie (FACS)

Produktnummer: ABIN132134

Produktbeschreibung

Weitere Bezeichnung: CD68

Format: Purified

Isotyp: IgG1

Spezifität: CD68 (macrosialin) is a type-one membrane protein with significant sequence homology to a family of lysosomal-associated glycoproteins (lamp). CD68 molecules are predominantly located intracellularily in cytoplasmic granules but can also be detected in smaller amounts at the cell surface. Particular strong intracellular expression is observed for monocytes and macrophages. In addition, Langerhans cells as well as plasmacytoid dendritic cells express clear-cut levels of CD68. Low-level reactivity is also observed with a subset of B lymphocytes and activated T lymphocytes. Oxidized low-density lipoprotein is a ligand for CD68. The CD68 mAb (clone Ki-M7) reacts with human macrosialin.

Anwendungen

Applikationshinweise: The CD68 mAb permits the identification and enumeration of monocytes, Langerhans cells and plasmacytoid dendritic cells (in combination with CD4/CD56 staining) in normal and malignant human blood and bone marrow samples using flow cytometry. Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained which cannot be attributed to differences in laboratory procedures, please contact us. Samples: Biological fluids (blood, bone marrow, and others) must be collected under sterile conditions. Anticoagulation with EDTA or heparin is recommended. The samples should be stored at room temperature until used. For optimal results, samples should be processed and analyzed within 24 hours. Samples with high numbers of non-viable cells might cause false results, such cases require determination of cell viability with e.g. propidium iodide. All biological samples have to be handled with caution. Always consider them as potentially infective. Use appropriate precautions such as gloves, lab-coat, etc. Sensitivity: The sensitivity of CD68 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAbconcentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50?l of leukocytes containing 107cells/ml are stained with 20?l mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.

Reinigung: Chromatography

Buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3

Lagerung: For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

anti-CD68 (CD68) antibody (FITC)

Publikationen

Publikationen: Rabinowitz, Gordon: "Macrosialin, a macrophage-restricted membrane sialoprotein differentially glycosylated in response to inflammatory stimuli." in: The Journal of experimental medicine, Vol. 174, Issue 4, pp. 827-36, 1991 (PubMed).

Fukuda: "Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficking." in: The Journal of biological chemistry, Vol. 266, Issue 32, pp. 21327-30, 1991 (PubMed).

Davey, Cordell, Erber et al.: "Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages." in: Journal of clinical pathology, Vol. 41, Issue 7, pp. 753-8, 1988 (PubMed).

Pulford, Rigney, Micklem et al.: "KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections." in: Journal of clinical pathology, Vol. 42, Issue 4, pp. 414-21, 1989 (PubMed).

Scheinecker, Strobl, Fritsch et al.: "Granulomonocyte-associated lysosomal protein expression during in vitro expansion and differentiation of CD34+ hematopoietic progenitor cells." in: Blood, Vol. 86, Issue 11, pp. 4115-23, 1996 (PubMed).

Knapp, Strobl, Majdic: "Flow cytometric analysis of cell-surface and intracellular antigens in leukemia diagnosis." in: Cytometry, Vol. 18, Issue 4, pp. 187-98, 1995 (PubMed).

Ramprasad, Fischer, Witztum et al.: "The 94- to 97-kDa mouse macrophage membrane protein that recognizes oxidized low density lipoprotein and phosphatidylserine-rich liposomes is identical to macrosialin, the mouse homologue of human CD68." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 21, pp. 9580-4, 1995 (PubMed).

Strobl, Scheinecker, Csmarits et al.: "Flow cytometric analysis of intracellular CD68 molecule expression in normal and malignant haemopoiesis." in: British journal of haematology, Vol. 90, Issue 4, pp. 774-82, 1995 (PubMed).

Holness, Simmons: "Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins." in: Blood, Vol. 81, Issue 6, pp. 1607-13, 1993 (PubMed).

Ramprasad, Terpstra, Kondratenko et al.: "Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low density lipoprotein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 25, pp. 14833-8, 1997 (PubMed).

Strobl, Scheinecker, Riedl et al.: "Identification of CD68+lin- peripheral blood cells with dendritic precursor characteristics." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 2, pp. 740-8, 1998 (PubMed).

Feuillard, Jacob, Valensi et al.: "Clinical and biologic features of CD4(+)CD56(+) malignancies." in: Blood, Vol. 99, Issue 5, pp. 1556-63, 2002 (PubMed).

Sadovnikova, Parovichnikova, Savchenko et al.: "The CD68 protein as a potential target for leukaemia-reactive CTL." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 16, Issue 10, pp. 2019-26, 2002 (PubMed).

Strobl, Knapp: "Myeloid cell-associated lysosomal proteins as flow cytometry markers for leukocyte lineage classification." in: Journal of biological regulators and homeostatic agents, Vol. 18, Issue 3-4, pp. 335-9, 2005 (PubMed).