CD3 (CD3E) Antikörper (PE)

Antigen: CD3 (CD3E)

Synonyme: T3E, TCRE, FLJ18683

Klonalität: Monoklonal (UCHT1)

Wirt: Maus

Reaktivität: Human

Konjugat: PE

Applikation: Durchflusszytometrie (FACS), Immunfluoreszenz (IF)

Produktnummer: ABIN132116

Produktbeschreibung

Weitere Bezeichnung: CD3

Format: Purified

Isotyp: IgG1

Klon: UCHT1

Spezifität: UCHT1 is directed against human CD3

Anwendungen

Applikationshinweise: The UCHT1 antibody permits the identification and enumeration of normal and leukemic human blood and bone marrow cells using flow cytometry. Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained which cannot be attributed to differences in laboratory procedures, please contact us. Samples: Biological fluids (blood, bone marrow, and others) must be collected under sterile conditions. Anticoagulation with EDTA or heparin is recommended. The samples should be stored at room temperature until used. For optimal results, samples should be processed and analyzed within 24 hours. Samples with high numbers of non-viable cells might cause false results, such cases require determination of cell viability with e.g. propidium iodide. All biological samples have to be handled with caution. Always consider them as potentially infective. Use appropriate precautions such as gloves, lab-coat, etc. Sensitivity: The sensitivity of UCHT1 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, an mAbconcentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ?l of leukocytes containing 107cells/ml are stained with 20 ?l mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.

Lagerung: For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

anti-CD3 (CD3E) antibody (PE)

Publikationen

Publikationen: Catovsky, Matutes, Buccheri et al.: "A classification of acute leukaemia for the 1990s." in: Annals of hematology, Vol. 62, Issue 1, pp. 16-21, 1991 (PubMed).

Janossy, Coustan-Smith, Campana: "The reliability of cytoplasmic CD3 and CD22 antigen expression in the immunodiagnosis of acute leukemia: a study of 500 cases." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 3, Issue 3, pp. 170-81, 1989 (PubMed).

Rani, De Oliveira, Catovsky: "Different expression of CD3 and CD22 in leukemic cells according to whether tested in suspension or fixed on slides." in: Hematologic pathology, Vol. 2, Issue 2, pp. 73-8, 1989 (PubMed).

Campana, Thompson, Amlot et al.: "The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 2, pp. 648-55, 1987 (PubMed).

Clevers, Alarcon, Wileman et al.: "The T cell receptor/CD3 complex: a dynamic protein ensemble." in: Annual review of immunology, Vol. 6, pp. 629-62, 1988 (PubMed).

Beverley, Linch, Callard: "Human Leucocyte antigens." in: Haematology and blood transfusion, Vol. 26, pp. 309-13, 1982 (PubMed).

Burns, Boyd, Beverley: "Two monoclonal anti-human T lymphocyte antibodies have similar biologic effects and recognize the same cell surface antigen." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 129, Issue 4, pp. 1451-7, 1982 (PubMed).

Groeneveld, te Marvelde, van den Beemd et al.: "Flow cytometric detection of intracellular antigens for immunophenotyping of normal and malignant leukocytes." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 10, Issue 8, pp. 1383-9, 1996 (PubMed).

Lanza, Latorraca, Moretti et al.: "Comparative analysis of different permeabilization methods for the flow cytometry measurement of cytoplasmic myeloperoxidase and lysozyme in normal and leukemic cells." in: Cytometry, Vol. 30, Issue 3, pp. 134-44, 1997 (PubMed).

Braylan, Orfao, Borowitz et al.: "Optimal number of reagents required to evaluate hematolymphoid neoplasias: results of an international consensus meeting." in: Cytometry, Vol. 46, Issue 1, pp. 23-7, 2001 (PubMed).

Van, Schoonjans, Struijk et al.: "Influence of dialysate on gastric emptying time in peritoneal dialysis patients." in: Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis, Vol. 22, Issue 1, pp. 32-8, 2002 (PubMed).

van SANDE, van ROS, DRUET: "Determination of haptoglobin-groups frequencies by starch-gel and agar-gel electrophoresis: application of Belgian and Barundi populations." in: Nature, Vol. 197, pp. 603-4 (PubMed).

Paietta: "How to optimize multiparameter flow cytometry for leukaemia/lymphoma diagnosis." in: Best practice & research. Clinical haematology, Vol. 16, Issue 4, pp. 671-83, 2003 (PubMed).