S-Tag Antikörper

Antigen: S-Tag

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN132044

Produktbeschreibung

Immunogen: This antibody was purified from rabbit serum using affinity chromatography. The rabbit was immunized with carrier protein (CP) conjugated to a synthetic peptide, CP –KETAAAKFERQHIDSC.

Beschreibung: Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins ex- pressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to pro- duce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. S-tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ri- bonuclease A. S-Tag can combine with S-protein to form functional RNase S, allowing detection and quantification of S-tag fusion proteins by enzymatic assays. However, antibody against the S-Tag sequence provides a more con- venient and flexible method to easily identify, detect, or purify S-tag containing fusion proteins.

Spezifität: This antibody reacts with S-Tag on Western blotting.

Anwendungen

Applikationshinweise: Western blotting: 1 µg / ml for chemiluminescence detection system. Immunoprecipitation: 5 µg / 200 µL of E. coli lysate. Immunohistochemistry: Not tested.

Konzentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL PBS con- taining 50% glycerol, pH 7.2. Contains no preservatives.

Forschungsgebiet: Tags/Labels

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunoprecipitation of S-Tag from BL21/pET30a-HRF E. coli lysate with rabbit IgG (1) and anti-S-Tag-Agarose (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with ABIN132042