Kaede Antikörper

Antigen: Kaede

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Western Blot (WB)

Produktnummer: ABIN132028

Produktbeschreibung

Immunogen: This antibody is a rabbit polyclonal antibody against the full length CoralHue Kaede expressed protein. The IgG was fractioned by salting out and gel filtration on a Sephadex G-200 column.

Beschreibung: CoralHue™ Kaede protein emits bright green fluorescence that can be irreversibly photoconverted to red. The green-to-red conversion is highly sensitive to irradiation with UV or violet light (350-410 nm). Maximal illumination results in a 2,000-fold increase in the ratio of red-to-green signal. The excitation lights used to elicit red and green fluorescence do not induce the photoconversion. This property provides a simple and powerful technique for regional optical marking.

Spezifität: This antibody reacts with CoralHue Kaede protein by Western blotting.

Anwendungen

Applikationshinweise: SDS-PAGE & Western Blotting 1) Wash the cells 3 times with PBS and suspend with 10 volumes of cold Lysis buffer (50 mM Tris-HCl, pH 7.2, 250 mM NaCl, 0.1% NP-40, 2 mM EDTA, 10% glycerol) containing appropriate protease inhibitors. Incubate it at 4 o C with rotating for 30 minutes, then sonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4 o C and transfer the supernatant to another tube. Measure the protein concentration of the supernatant and add the cold Lysis buffer to make 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli’s sample buffer. 4) Boil the samples for 2 minutes and centrifuge. Load 10 µL of the sample per lane in a 1 mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) 250 150 100 75 50 37 25 20 kD 250 150 100 75 50 37 25 20 kD 250 150 100 75 50 37 25 20 kD Western blot detection of CoralHue Kaede expressed in 293T cells. Left , Immunoblotted with Western blotting: 5 µg / mL for chemiluminescence detection system. Immunoprecipitation: Not tested. Immunohistochemistry: Not tested. Immunocytochemistry: Not tested. Flow Cytometry: Not tested.

Konzentration: 5 mg/mL

Buffer: 500 µg IgG in 100 µL volume of 10mM phosphate buffer containing 0.3M NaCl and 0.1 % NaN 3 pH 8.0. *Azide may react with copper or lead in plumbing systems to form explosive metal azides. Therefore, always flush drain thoroughly with excess water when disp

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot detection of CoralHue Kaede expressed in 293T cells. Left , Immunoblotted with ABIN132027Right , Immunoblotted with Normal rabbit IgG