Glutamic Acid Decarboxylase (GAD) Antikörper

Antigen: Glutamic Acid Decarboxylase (GAD)

Synonyme: GAD, Gad1, Ci-GAD

Klonalität: Monoklonal (9A6)

Wirt: Maus

Reaktivität: Human, Maus

Applikation: Western Blot (WB), Immunhistochemie (IHC)

Produktnummer: ABIN131839

Produktbeschreibung

Immunogen: This antibody was purified from mouse ascites fluid using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with the recombinant GST-human brain GAD 65 fusion protein corresponding to full-length amino acids (1-585 a.a.).

Format: Liquid

Isotyp: IgG1, IgG1 kappa  (Passende Sekundärantikörper)

Klon: 9A6

Beschreibung: Glutamic acid decarboxylase (GAD) catalyzes formation of .-Aminobutyric acid (GABA), which is an inhibitory neurotransmitter, and is present in brain as well as several tissues outside the central nervous system. Biological functions of GAD and GABA extend beyond regulation of neurotransmission to include effects on the immune system as well as modulation of cell proliferation, protein synthesis, and metabolism. Two isoforms of GAD, GAD 65 and GAD 67 , each derived from a single separate gene, were isolated from human fetal brain .gt11 cDNA library.

Spezifität: This antibody reacts with GAD 65 and GAD 67 on Western blotting and Immunohistochemistry in murine and rat samples. This antibody detects 65 kDa and 67 kDa GAD by Western blotting using mouse and rat total brain cell lysate.

Anwendungen

Applikationshinweise: Western blotting: 1 µg/mL for chemiluminescence detection system. Immunoprecipitation: Not tested. Immunohistochemistry: 1-10 µg/mL

Konzentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL PBS containing 50% glycerol, pH 7.2. Contains no preservatives.

Forschungsgebiet: Enzyme, Metabolismus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of GAD expression in mouse (1) and rat (2) brain using ABIN131839.

Human Pancreas

Publikationen

Publikationen: DeAizpurua, Harrison, Cram: "An ELISA for antibodies to recombinant glutamic acid decarboxylase in IDDM." in: Diabetes, Vol. 41, Issue 9, pp. 1182-7, 1992 (PubMed).

Bu, Erlander, Hitz et al.: "Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 6, pp. 2115-9, 1992 (PubMed).

Karlsen, Hagopian, Grubin et al.: "Cloning and primary structure of a human islet isoform of glutamic acid decarboxylase from chromosome 10." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 19, pp. 8337-41, 1991 (PubMed).

Michelsen, Petersen, Boel et al.: "Cloning, characterization, and autoimmune recognition of rat islet glutamic acid decarboxylase in insulin-dependent diabetes mellitus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 19, pp. 8754-8, 1991 (PubMed).

Grubin, Daniels, Toivola et al.: "A novel radioligand binding assay to determine diagnostic accuracy of isoform-specific glutamic acid decarboxylase antibodies in childhood IDDM." in: Diabetologia, Vol. 37, Issue 4, pp. 344-50, 1994 (PubMed).

Daw, Ujihara, Atkinson et al.: "Glutamic acid decarboxylase autoantibodies in stiff-man syndrome and insulin-dependent diabetes mellitus exhibit similarities and differences in epitope recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 2, pp. 818-25, 1996 (PubMed).

Kasuga, Maruyama, Ozawa et al.: "Antibody to the M(r) 65,000 isoform of glutamic acid decarboxylase are detected in non-insulin-dependent diabetes in Japanese." in: Journal of autoimmunity, Vol. 9, Issue 1, pp. 105-11, 1996 (PubMed).

Kuwajima, Nishimura, Yoshikawa: "Necdin promotes GABAergic neuron differentiation in cooperation with Dlx homeodomain proteins." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 20, pp. 5383-92, 2006 (PubMed).