Nestin (NES) Antikörper

Antigen: Nestin (NES)

Synonyme: FLJ21841, Nbla00170, C78523, ESTM46, AA166324, NES, paranemin

Klonalität: Monoklonal (10C2)

Wirt: Maus

Applikation: Western Blot (WB), Immunhistochemie (IHC), Durchflusszytometrie (FACS)

Produktnummer: ABIN131779

Produktbeschreibung

Weitere Bezeichnung: Nestin

Immunogen: This antibody was purified from hybridoma (clone 10C2) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3-X63-Ag8-653 with Balb/c mouse splenocyte immunized with full length human recombinant Nestin.

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 10C2

Beschreibung: Nestin is a large (220~240kDa) Class VI intermediate filament that is abundantly expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments. Since nestin is absent from nearly all mature CNS cells, it is commonly used as a marker for neuronal stem cells. Nestin is also expressed in gliomas, melanoma, and tumor endothelial cells, making it an excellent angiogenic marker to evaluate neovascularity of endothelial cells in tumors.

Spezifität: This antibody reacts with human Nestin (220-240kDa) on Western blotting.

Anwendungen

Applikationshinweise: Western blotting: 1 µg / mL for chemiluminescence detection system. Immunohistochemistry: Under evaluation Heat treatment is necessary for paraffin embedded sections. Microwave oven: 2 times for 10 minutes each in citrate buffer (pH 6.5). Immunocytochemistry: Not tested. Immunoprecipitation: Not recommended. Flow Cytometry: 10 µg / ml (final concentration)

Konzentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of Human Nestin expression in U251 cells (1), SK-N-SH cells (2), Jurkat cells (3), Raji cells (4), Mouse brain (5), Rat brain (6) and PC12 cells (7) using ABIN131779.

Western blot analysis of Human Nestin expression in U87/MG cells (1), HeLa cells (2) and ZR75-1 cells (3) using ABIN131779.

Flow cytometric analysis of Human Nestin expression in SK-N-SH cells (right) and Jurkat cells (left). Open histograms indicate the reaction of isotypic control to the cells. Shaded histograms indicate the reaction of ABIN131779 to the cells.