Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) Antikörper

Antigen: Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27)

Synonyme: APC3, HNUC, CDC27Hs, D0S1430E, D17S978E, wu:fb54g06, wu:fc88c12, AI452358, BC023187, MGC80529, CDC27, MGC145551, NV10210

Klonalität: Monoklonal (AF3-1)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus), Rind (Kuh), Xenopus laevis

Applikation: Western Blot (WB), Immunpräzipitation (IP), Immunzytochemie (ICC), ELISA, Immunhistochemie (IHC)

Produktnummer: ABIN131716

Produktbeschreibung

Weitere Bezeichnung: Cdc27

Immunogen: This antibody was purified from hybridoma (clone AF3.1) supernatant using protein A agarose beads. This hybridoma was established by fusion of mouse myeloma cell P3-X63-Ag8 with Balb/c mouse splenocyte immunized with a human CDC27 peptide C-terminal (814aa-823aa).

Isotyp: IgG1, IgG2b  (Passende Sekundärantikörper)

Klon: AF3-1

Beschreibung: CDC16, CDC27 and CDC34 are components of the APC (anaphase-promoting complex) that also play a role as components of the ubiquitin- conjugating E3 enzyme. A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B, resulting in cyclin B/Cdk complex degradation. CDC27 is therefore required for progression of the cell cycle, where it executes essential mitotic functions near the metaphase/anaphase transition. CDC27 is ~97 kDa and is localized to the centrosome and mitotic spindle.

Spezifität: This antibody reacts with 97 kDa membrane protein CDC27 on Western blotting.

Anwendungen

Applikationshinweise: Western blotting: 1 µg / mL for chemiluminescence detection system. Immunoprecipitation: 2 µg / 200 µL of cell extract. Immunohistochemistry: 10 µg / mL Heat treatment is necessary for paraffin embedded sections. Autoclave: 10 minutes at 110 o C in citrate buffer (pH 6.5). Immunocytochemistry : Not recommended. Flow Cytometry: Not recommended.

Konzentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. Contains no preservatives.

Forschungsgebiet: Zellzyklus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunoprecipitation of CDC27 from Raji cells with ABIN131716 (1) or normal mouse IgG (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with ABIN131716.

Western blot analysis of CDC27 expression in Jurkat cells (1), Raji cells (2), K562 cells (3), HL-60 cells (4) and MCF-7 cells (5) using ABIN131716.

Publikationen

Publikationen: Yamano, Tsurumi, Gannon et al.: "The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor." in: The EMBO journal, Vol. 17, Issue 19, pp. 5670-8, 1998 (PubMed).

Zur, Brandeis: "Timing of APC/C substrate degradation is determined by fzy/fzr specificity of destruction boxes." in: The EMBO journal, Vol. 21, Issue 17, pp. 4500-10, 2002 (PubMed).