Syntaxin 1 (STX1) Antikörper

Antigen: Syntaxin 1 (STX1)

Synonyme: CG10716, CG18615, CG5448, CT30033, Dm Syx1, DmSyx1A, SYX1A, Syt1A, Syx, Syx1, SyxA, anon-EST:Gibbs4, anon-WO02059370.54, dSyx1, l(3)06737, synt, syt-1A, syx-1, syx-1A, DmelCG31136, CG31136

Klonalität: Monoklonal (HPC-1-STX-01)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus), Rind (Kuh), Kaninchen

Applikation: Western Blot (WB), Immunpräzipitation (IP), Immunhistochemie (IHC)

Produktnummer: ABIN131691

Produktbeschreibung

Weitere Bezeichnung: Syntaxin-1

Immunogen: This antibody was purified from hybridoma (clone HPC-1) supernatant using protein A agarose beads. This hybridoma was established by fusion of mouse myeloma cell NS1 with Balb/c mouse splenocyte immunized with a synaptosomal plasma membrane fraction from adult rat hippocampus.

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: HPC-1-STX-01

Beschreibung: Syntaxin 1, also known as HPC-1, is a 35 kDa integral membrane protein that is abundantly expressed in neurons and neuroendocrine cells. Syntaxin is essential for synaptic vesicle fusion and it interacts with several other proteins important for synaptic function, including its partners in the fusion complex (Synaptobrevin/VAMP, SNAP-25, a-SNAP, synaptotagmin, Munc-18/n-Sec1, and Ca2+ - channels). Syntaxin is concentrated in synapses and synaptic vesicles where it is a component of the SNARE complex.

Spezifität: This antibody reacts with 35 kDa membrane protein syntaxin-1, expressed predominantly in neuronal tissues on Western blotting.

Anwendungen

Applikationshinweise: Western blotting: 0.1 µg/mL for chemiluminescence detection system. Immunoprecipitation: 2 µg / 200 µL of cell extract. Immunohistochemistry: 5 µg/mL Heat treatment is necessary for paraffin embedded sections. Microwave oven: 2 times for 10 minutes each in citrate buffer (pH 6.5)

Konzentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunoprecipitation of syntaxin-1 from mouse brain extracts with ABIN131691 (1) or mouse IgG1 (2). After immunoprecipitation with the antibody, the immunocomplex was resolved on SDS-PAGE and immunoblotted with ABIN131691.

Western blot analysis of syntaxin-1 expression in mouse brain extracts (1) and PC12 cells (2) using ABIN131691.

Immunohistochemical detection of syntaxin-1 on paraffin embedded section of a human spinal cord with ABIN131691.

Publikationen

Publikationen: Galli, McPherson, De Camilli: "The V0 sector of the V-ATPase, synaptobrevin, and synaptophysin are associated on synaptic vesicles in a Triton X-100-resistant, freeze-thawing sensitive, complex." in: The Journal of biological chemistry, Vol. 271, Issue 4, pp. 2193-8, 1996 (PubMed).

Yang, Larsson, Braenstroem et al.: "Syntaxin 1 interacts with the L(D) subtype of voltage-gated Ca(2+) channels in pancreatic beta cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 18, pp. 10164-9, 1999 (PubMed).

Foletti, Lin, Finley et al.: "Phosphorylated syntaxin 1 is localized to discrete domains along a subset of axons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 20, Issue 12, pp. 4535-44, 2000 (PubMed).

Graff, Castrop, Bauer et al.: "Expression of vesicular monoamine transporters, synaptosomal-associated protein 25 and syntaxin1: a signature of human small cell lung carcinoma." in: Cancer research, Vol. 61, Issue 5, pp. 2138-44, 2001 (PubMed).