CD6 (4F2) Antikörper (FITC)

Antigen: CD6 (4F2)

Klonalität: Monoklonal (4F2)

Wirt: Maus

Reaktivität: Human

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN131585

Produktbeschreibung

Immunogen: This antibody was purified from hybridoma (clone 4F2) supernatant using Protein-A Sepharose. This hybridoma was established by fusion of mouse myelo ma cell P3x63/Ag8 with Balb/c mouse splenocyte immunized with COS7 cells transfected with CD6.

Format: Liquid

Isotyp: IgG1 kappa

Klon: 4F2

Spezifität: This antibody reacts with human CD6 SRCR (Scavenger Receptor Cysteine Rich) domain 1.

Anwendungen

Applikationshinweise: Flow cytometric analysis for floating cells . Protocol 1: We use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN 3 ]. 2) Resuspend the cells with washing buffer (5x10 6 cells/mL). 3) Add 50 µL of the cell suspension into each tube, and centrifuge at 500xg for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration. 4) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0.1% NaN 3 to the cell pellet after tapping. Mix well, and incubate for 5 minutes at room temperature (20~25 o C). 5) Add 30 µL of the FITC labeled CD6 (4F2) monoclonal antibody (10-20 µg/mL) diluted with the washing buffer. Mix well, and incubate for 30 minutes at room temperature (20~25 o C). 6) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. . Protocol 2: We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN 3 ]. 2) Resuspend the cells with PBS containing 25% normal goat serum, 1 mg/mL normal human IgG and 0.1% NaN 3 (5x10 6 cells/mL). 3) Add 20 µL of the non diluted FITC labeled CD6 (4F2) monoclonal antibody (50 µg/mL) into each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutes at room temperature (20~25 o C). 5) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Flow cytometry: 10-20 µg/mL (final concentration) Research Applications.

Buffer: 50 µg IgG in 1 mL volume of PBS containing 1% BSA and 0.1% NaN 3 . * Azide may react with copper or lead in plumbing system to form explosive metal azides. Therefore, always flush plenty of water when disposing materials containing azide into drain.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Patel: "CD6." in: Journal of biological regulators and homeostatic agents, Vol. 14, Issue 3, pp. 234-6, 2001 (PubMed).