HA-Tag Antikörper
| Antigen |
|
| Klonalität |
Monoklonal (2C16) |
|
Wirt
|
|
|
Applikation
|
Immunfluoreszenz (IF) (65), Durchflusszytometrie (FACS) (55), Western Blot (WB) (53), ELISA (29), Immunpräzipitation (IP) (28), Immunzytochemie (ICC) (6), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (6), Immunhistochemie (Paraffinschnitte) (IHC (p)) (6), Dot Blot (Dot) (5), Immunoelectron Microscopy (IEM) (3), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (2), Chromatin-Immunopräzipitation (ChiP) (1), Immunhistochemie (IHC) (1), Immunostaining (ISt) (1)
|
| Produktnummer |
ABIN131279 |
| Menge |
100 µg (Varianten) |
| Preis |
265,00 € Zzgl. Versandkosten €20,00, €20,00 Trockeneispauschale sowie MWSt
|
| Lieferung nach |
|
| Verfügbarkeit |
Lieferung in 10 Werktagen |
|
Isotyp
|
IgG1 (Passende Sekundärantikörper)
|
|
Klon
|
2C16
|
|
Beschreibung
|
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immuno- precipitation, and immunostaining techniques. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.
|
|
Spezifität
|
The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected mammalian cells.
|
|
Applikationshinweise
|
The Anti-HA-FITC antibody can be used for one-step localization of recombinant HA-tagged proteins by standard immunofluorescence methods (2-10 µg/ml). However, the optimal conditions should be determined individually.
|
|
Buffer
|
Supplied as 100 µg (0.2 mg/ml) anti-HA-FITC monoclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% sodium azide.
|
|
Forschungsgebiet
|
Tags/Labels
|
|
Beschränkungen
|
Nur für Forschungszwecke einsetzbar
|
|
Publikationen
|
Field, Nikawa, Broek et al.: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).
|
Alternativen zu Antigen "HA-Tag", Typ "Antikörper" finden
|
Wirte
|
Kaninchen (50), Maus (49), Ziege (6)
|
|
Reaktivitäten
|
Fusion Protein (Tag) (42), Alle Spezies (15), Tag (14), Maus (4), Human (3), Ratte (Rattus) (3), Diverse Spezies (1)
|
|
Applikationen
|
Immunfluoreszenz (IF) (65), Durchflusszytometrie (FACS) (55), Western Blot (WB) (53), ELISA (29), Immunpräzipitation (IP) (28), Immunzytochemie (ICC) (6), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (6), Immunhistochemie (Paraffinschnitte) (IHC (p)) (6), Dot Blot (Dot) (5), Immunoelectron Microscopy (IEM) (3), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (2), Chromatin-Immunopräzipitation (ChiP) (1), Immunhistochemie (IHC) (1), Immunostaining (ISt) (1)
|
|
Konjugate
|
Biotin (6), HRP (6), FITC (4), Alexa Flour 488 (3), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), Gold (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), RBITC (3)
|
Alternativen 
